miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
16262 3' -55.1 NC_004084.1 + 1515 0.69 0.491927
Target:  5'- -cGACGaggacCUCUACACcGCUGUCGAc -3'
miRNA:   3'- uaCUGCga---GAGGUGUGuCGACAGCUu -5'
16262 3' -55.1 NC_004084.1 + 28765 0.66 0.679684
Target:  5'- cAUGuaccaGCUgUCCGCGCGGUcGUCGAc -3'
miRNA:   3'- -UACug---CGAgAGGUGUGUCGaCAGCUu -5'
16262 3' -55.1 NC_004084.1 + 29607 0.67 0.623607
Target:  5'- -cGAUGCUCgcCCACAacggcaccUGGCUGUCGGc -3'
miRNA:   3'- uaCUGCGAGa-GGUGU--------GUCGACAGCUu -5'
16262 3' -55.1 NC_004084.1 + 37545 0.66 0.657316
Target:  5'- -aGACGCUCgauUCCAuCGcCGGCgcgGUCGAc -3'
miRNA:   3'- uaCUGCGAG---AGGU-GU-GUCGa--CAGCUu -5'
16262 3' -55.1 NC_004084.1 + 39835 0.68 0.53475
Target:  5'- gAUGGCGCUCgagUCCAUcggcggccGCGGCgccGUCGAGc -3'
miRNA:   3'- -UACUGCGAG---AGGUG--------UGUCGa--CAGCUU- -5'
16262 3' -55.1 NC_004084.1 + 51865 1.04 0.0018
Target:  5'- cAUGACGCUCUCCACACAGCUGUCGAAa -3'
miRNA:   3'- -UACUGCGAGAGGUGUGUCGACAGCUU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.