Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16262 | 5' | -52 | NC_004084.1 | + | 202 | 0.65 | 0.904086 |
Target: 5'- gUUCCGgGagcccgcacggccaUCGGCGggGGAg-UCGUCg -3' miRNA: 3'- aGAGGCgC--------------AGUCGCuuCUUgaAGCAG- -5' |
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16262 | 5' | -52 | NC_004084.1 | + | 56189 | 0.66 | 0.90202 |
Target: 5'- aUCUCCGCGcuggccgaagcgagCGGCGAGGA-CaUCGa- -3' miRNA: 3'- -AGAGGCGCa-------------GUCGCUUCUuGaAGCag -5' |
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16262 | 5' | -52 | NC_004084.1 | + | 50246 | 0.66 | 0.90202 |
Target: 5'- cCUCUGCGUCcucgucgccguuggcGGCGAGGucCUcgaCGUCu -3' miRNA: 3'- aGAGGCGCAG---------------UCGCUUCuuGAa--GCAG- -5' |
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16262 | 5' | -52 | NC_004084.1 | + | 36455 | 0.66 | 0.899228 |
Target: 5'- --aCCGUGUCgaucucGGCGGAGAcg-UCGUCc -3' miRNA: 3'- agaGGCGCAG------UCGCUUCUugaAGCAG- -5' |
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16262 | 5' | -52 | NC_004084.1 | + | 32622 | 0.66 | 0.892053 |
Target: 5'- aCUCCaGCGau-GCGGAGGACgagacggUCGUUg -3' miRNA: 3'- aGAGG-CGCaguCGCUUCUUGa------AGCAG- -5' |
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16262 | 5' | -52 | NC_004084.1 | + | 8813 | 0.66 | 0.868913 |
Target: 5'- aUCUCCGuCGaCGGCGAacucgccaAGuACcUCGUCg -3' miRNA: 3'- -AGAGGC-GCaGUCGCU--------UCuUGaAGCAG- -5' |
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16262 | 5' | -52 | NC_004084.1 | + | 20870 | 0.66 | 0.868913 |
Target: 5'- --aCCGCGcCGGCGAuGGAAUcgagCGUCu -3' miRNA: 3'- agaGGCGCaGUCGCU-UCUUGaa--GCAG- -5' |
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16262 | 5' | -52 | NC_004084.1 | + | 14374 | 0.66 | 0.868102 |
Target: 5'- aUCUUCGCGcaccucccacUCGGCGAgaucGGGGCgauguucucgaagUUCGUCg -3' miRNA: 3'- -AGAGGCGC----------AGUCGCU----UCUUG-------------AAGCAG- -5' |
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16262 | 5' | -52 | NC_004084.1 | + | 48925 | 0.67 | 0.860683 |
Target: 5'- cUUCCG-GUCGGCGccGGGCUUCa-- -3' miRNA: 3'- aGAGGCgCAGUCGCuuCUUGAAGcag -5' |
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16262 | 5' | -52 | NC_004084.1 | + | 57249 | 0.67 | 0.834543 |
Target: 5'- --aCCGCGUCGGCGAuGAcGCgaaggUgGUCu -3' miRNA: 3'- agaGGCGCAGUCGCUuCU-UGa----AgCAG- -5' |
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16262 | 5' | -52 | NC_004084.1 | + | 51259 | 0.67 | 0.834543 |
Target: 5'- --gCUuCGUCAGCGgcGAACgcugugUCGUCg -3' miRNA: 3'- agaGGcGCAGUCGCuuCUUGa-----AGCAG- -5' |
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16262 | 5' | -52 | NC_004084.1 | + | 34848 | 0.69 | 0.734743 |
Target: 5'- gCUCuCGaCGaCAGCGggGAGCgcgauaUCGUCc -3' miRNA: 3'- aGAG-GC-GCaGUCGCuuCUUGa-----AGCAG- -5' |
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16262 | 5' | -52 | NC_004084.1 | + | 55539 | 0.7 | 0.702184 |
Target: 5'- cCUCCGCGaUCGcgcuggcuggccGCGAcGAACU-CGUCg -3' miRNA: 3'- aGAGGCGC-AGU------------CGCUuCUUGAaGCAG- -5' |
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16262 | 5' | -52 | NC_004084.1 | + | 21874 | 0.7 | 0.680104 |
Target: 5'- --aCUGCGUCAuccgcaucGCGAGGAACUUCa-- -3' miRNA: 3'- agaGGCGCAGU--------CGCUUCUUGAAGcag -5' |
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16262 | 5' | -52 | NC_004084.1 | + | 28398 | 0.72 | 0.590777 |
Target: 5'- aUCUUCGCGUCGauGCGAAcgcuGACgUCGUCa -3' miRNA: 3'- -AGAGGCGCAGU--CGCUUc---UUGaAGCAG- -5' |
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16262 | 5' | -52 | NC_004084.1 | + | 34996 | 0.73 | 0.524977 |
Target: 5'- cCUgCGUGUCAGCGAucagcuGGGCUcgaguuUCGUCg -3' miRNA: 3'- aGAgGCGCAGUCGCUu-----CUUGA------AGCAG- -5' |
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16262 | 5' | -52 | NC_004084.1 | + | 54081 | 0.74 | 0.482675 |
Target: 5'- cCUCgGCGUCGGUGGAGAACgcucCGg- -3' miRNA: 3'- aGAGgCGCAGUCGCUUCUUGaa--GCag -5' |
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16262 | 5' | -52 | NC_004084.1 | + | 51898 | 1.11 | 0.001641 |
Target: 5'- uUCUCCGCGUCAGCGAAGAACUUCGUCa -3' miRNA: 3'- -AGAGGCGCAGUCGCUUCUUGAAGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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