Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16263 | 3' | -57.5 | NC_004084.1 | + | 11109 | 0.66 | 0.635725 |
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Target: 5'- cGCCgUUCGcggcucgaAGGAUGUCCCagaGCCGcuGGAc -3' miRNA: 3'- aCGG-AAGC--------UCUUGCAGGGg--CGGC--UCU- -5' |
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| 16263 | 3' | -57.5 | NC_004084.1 | + | 42406 | 0.66 | 0.64647 |
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Target: 5'- cGUCUUUGAGcucguuGAgGUCCCCGuuG-GAu -3' miRNA: 3'- aCGGAAGCUC------UUgCAGGGGCggCuCU- -5' |
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| 16263 | 3' | -57.5 | NC_004084.1 | + | 35926 | 0.66 | 0.64647 |
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Target: 5'- cGCgUUcaCGAGcAUGUCCCCggcgucGCCGGGGu -3' miRNA: 3'- aCGgAA--GCUCuUGCAGGGG------CGGCUCU- -5' |
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| 16263 | 3' | -57.5 | NC_004084.1 | + | 26968 | 0.66 | 0.64647 |
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Target: 5'- cUGCCguccUCGAGGGCaGUCaCCCugaaCGAGAc -3' miRNA: 3'- -ACGGa---AGCUCUUG-CAG-GGGcg--GCUCU- -5' |
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| 16263 | 3' | -57.5 | NC_004084.1 | + | 43740 | 0.66 | 0.64647 |
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Target: 5'- gGCCaaUCGAGGA---CCCgGCCGGGAc -3' miRNA: 3'- aCGGa-AGCUCUUgcaGGGgCGGCUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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