Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16263 | 3' | -57.5 | NC_004084.1 | + | 53340 | 0.69 | 0.459098 |
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Target: 5'- cGUCUUCGAGGuggacuGCGUCgucuacUCCGCCGAc- -3' miRNA: 3'- aCGGAAGCUCU------UGCAG------GGGCGGCUcu -5' |
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| 16263 | 3' | -57.5 | NC_004084.1 | + | 53434 | 0.73 | 0.262714 |
|
Target: 5'- aUGCC--CGAGuGgGUCCCCGCCGAu- -3' miRNA: 3'- -ACGGaaGCUCuUgCAGGGGCGGCUcu -5' |
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| 16263 | 3' | -57.5 | NC_004084.1 | + | 55234 | 0.67 | 0.539911 |
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Target: 5'- cGCC-UCGuucACGUCCUCGCCGucGAc -3' miRNA: 3'- aCGGaAGCucuUGCAGGGGCGGCu-CU- -5' |
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| 16263 | 3' | -57.5 | NC_004084.1 | + | 57109 | 0.7 | 0.385181 |
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Target: 5'- cGCCUcgauccagUCGAGGACGUCCUcgguguCGCCGu-- -3' miRNA: 3'- aCGGA--------AGCUCUUGCAGGG------GCGGCucu -5' |
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| 16263 | 3' | -57.5 | NC_004084.1 | + | 57811 | 0.69 | 0.449441 |
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Target: 5'- aGCUUcaacgaucaacUCGAGGGCGUCCUCaCCGaAGAg -3' miRNA: 3'- aCGGA-----------AGCUCUUGCAGGGGcGGC-UCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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