Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16264 | 3' | -57.4 | NC_004084.1 | + | 3447 | 0.66 | 0.661896 |
Target: 5'- uGGUgaccugUCGGAUCGCGagcgcCGGGGGaauaCGGAa -3' miRNA: 3'- gCCA------AGCCUAGCGCa----GCUCCUc---GCCU- -5' |
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16264 | 3' | -57.4 | NC_004084.1 | + | 45539 | 0.66 | 0.660835 |
Target: 5'- cCGGgaUGGGUUgGCG-CGAGGAccucgaaGCGGAg -3' miRNA: 3'- -GCCaaGCCUAG-CGCaGCUCCU-------CGCCU- -5' |
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16264 | 3' | -57.4 | NC_004084.1 | + | 49895 | 0.66 | 0.651276 |
Target: 5'- aGG-UCGGGUacagCGCGUagaUGAGGuucuGCGGAu -3' miRNA: 3'- gCCaAGCCUA----GCGCA---GCUCCu---CGCCU- -5' |
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16264 | 3' | -57.4 | NC_004084.1 | + | 19839 | 0.67 | 0.587522 |
Target: 5'- gCGGa--GGGUgacuucCGCGUCGAGG-GCGGu -3' miRNA: 3'- -GCCaagCCUA------GCGCAGCUCCuCGCCu -5' |
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16264 | 3' | -57.4 | NC_004084.1 | + | 23374 | 0.68 | 0.535263 |
Target: 5'- -cGUUCGGAuccgUCGCGagccacUCGAGGA-CGGAc -3' miRNA: 3'- gcCAAGCCU----AGCGC------AGCUCCUcGCCU- -5' |
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16264 | 3' | -57.4 | NC_004084.1 | + | 28104 | 0.69 | 0.474894 |
Target: 5'- aCGGUUCGGGgugCGCcUCGAcGAGCGc- -3' miRNA: 3'- -GCCAAGCCUa--GCGcAGCUcCUCGCcu -5' |
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16264 | 3' | -57.4 | NC_004084.1 | + | 31151 | 0.7 | 0.436591 |
Target: 5'- gCGGUUCGGAcCGCGagaucUCGucGAGCGcGGu -3' miRNA: 3'- -GCCAAGCCUaGCGC-----AGCucCUCGC-CU- -5' |
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16264 | 3' | -57.4 | NC_004084.1 | + | 30698 | 0.71 | 0.374091 |
Target: 5'- -aGUUCgagcaGGAgCGCGUCGAGGAGCuGAu -3' miRNA: 3'- gcCAAG-----CCUaGCGCAGCUCCUCGcCU- -5' |
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16264 | 3' | -57.4 | NC_004084.1 | + | 8199 | 0.71 | 0.354103 |
Target: 5'- gGGUUcgCGGAUgGCGUCGAGGuugaaccaguaguGCGGc -3' miRNA: 3'- gCCAA--GCCUAgCGCAGCUCCu------------CGCCu -5' |
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16264 | 3' | -57.4 | NC_004084.1 | + | 54055 | 0.72 | 0.317941 |
Target: 5'- aCGGUU-GGAcgCGCGaCGAGGAGaCGGGc -3' miRNA: 3'- -GCCAAgCCUa-GCGCaGCUCCUC-GCCU- -5' |
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16264 | 3' | -57.4 | NC_004084.1 | + | 50530 | 1.09 | 0.000878 |
Target: 5'- uCGGUUCGGAUCGCGUCGAGGAGCGGAg -3' miRNA: 3'- -GCCAAGCCUAGCGCAGCUCCUCGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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