miRNA: 3'- -ACAGGCAG-GUCUUG--------AGCAGGGucuCG- -5'
miRNA: 3'- -ACAGGcAGGUCU----UGAGC------------AGGGUCUCG- -5'
miRNA: 3'- acaGGCAGG-UCU-----UGAGcAGGGUCUCG- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16264 | 5' | -54.9 | NC_004084.1 | + | 51112 | 0.69 | 0.60056 |
|
Target: 5'- uUGUCCGUCuCGGAAUcgguguugUCGUCCUu--GCu -3' miRNA: 3'- -ACAGGCAG-GUCUUG--------AGCAGGGucuCG- -5' |
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| 16264 | 5' | -54.9 | NC_004084.1 | + | 53831 | 0.67 | 0.687756 |
|
Target: 5'- gUGUUCcUCCGGGaacgGCUCGaauccgaacucaUCCCGGAGUu -3' miRNA: 3'- -ACAGGcAGGUCU----UGAGC------------AGGGUCUCG- -5' |
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| 16264 | 5' | -54.9 | NC_004084.1 | + | 55092 | 0.69 | 0.557291 |
|
Target: 5'- --cCCGUCCgAGAcgcgcGCUCcUCCUGGAGCu -3' miRNA: 3'- acaGGCAGG-UCU-----UGAGcAGGGUCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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