Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16265 | 5' | -54.7 | NC_004084.1 | + | 53163 | 0.66 | 0.764756 |
Target: 5'- gACGAGGccgaugACUgaccccaGCGuGUUCGuCCGUCg -3' miRNA: 3'- -UGCUCCa-----UGAac-----CGCuCAAGC-GGCAG- -5' |
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16265 | 5' | -54.7 | NC_004084.1 | + | 41393 | 0.66 | 0.754501 |
Target: 5'- uCGAGGUAgagGGCGAGgagGCCGg- -3' miRNA: 3'- uGCUCCAUgaaCCGCUCaagCGGCag -5' |
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16265 | 5' | -54.7 | NC_004084.1 | + | 50148 | 0.66 | 0.754501 |
Target: 5'- gACGAGGUcgUUGuCGAGgaUGUCGUCa -3' miRNA: 3'- -UGCUCCAugAACcGCUCaaGCGGCAG- -5' |
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16265 | 5' | -54.7 | NC_004084.1 | + | 14040 | 0.66 | 0.733628 |
Target: 5'- aACGucGGGUGCUUGGCGAGacgaaGagGUCa -3' miRNA: 3'- -UGC--UCCAUGAACCGCUCaag--CggCAG- -5' |
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16265 | 5' | -54.7 | NC_004084.1 | + | 19591 | 0.66 | 0.733628 |
Target: 5'- cACGAGG-ACga--CGAGUUCGUgGUCa -3' miRNA: 3'- -UGCUCCaUGaaccGCUCAAGCGgCAG- -5' |
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16265 | 5' | -54.7 | NC_004084.1 | + | 46174 | 0.66 | 0.733628 |
Target: 5'- cGCGAacuugcccagcuGGUAggcGGCGAGaUCGUCGUCg -3' miRNA: 3'- -UGCU------------CCAUgaaCCGCUCaAGCGGCAG- -5' |
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16265 | 5' | -54.7 | NC_004084.1 | + | 36076 | 0.67 | 0.723033 |
Target: 5'- gACGAGG-ACcgcGaCGAGgUCGCCGUCu -3' miRNA: 3'- -UGCUCCaUGaacC-GCUCaAGCGGCAG- -5' |
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16265 | 5' | -54.7 | NC_004084.1 | + | 25468 | 0.67 | 0.712348 |
Target: 5'- uCGAGGUuCgcgGGCGgcgucagaaGGUgcgCGCCGUCc -3' miRNA: 3'- uGCUCCAuGaa-CCGC---------UCAa--GCGGCAG- -5' |
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16265 | 5' | -54.7 | NC_004084.1 | + | 55376 | 0.67 | 0.690751 |
Target: 5'- uCGAGGg---UGGCGAucaUCGCCGUa -3' miRNA: 3'- uGCUCCaugaACCGCUca-AGCGGCAg -5' |
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16265 | 5' | -54.7 | NC_004084.1 | + | 56606 | 0.68 | 0.635961 |
Target: 5'- gGCGucGUcGCcggucGGCGAGUUCGCCGg- -3' miRNA: 3'- -UGCucCA-UGaa---CCGCUCAAGCGGCag -5' |
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16265 | 5' | -54.7 | NC_004084.1 | + | 5692 | 0.69 | 0.60296 |
Target: 5'- uCGAGGUccGCUgGGuUGAGUUCGaggaCGUCg -3' miRNA: 3'- uGCUCCA--UGAaCC-GCUCAAGCg---GCAG- -5' |
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16265 | 5' | -54.7 | NC_004084.1 | + | 70 | 0.69 | 0.58107 |
Target: 5'- aACGAGGUugUgucGG-GAGUcguugugcuugUUGCCGUCu -3' miRNA: 3'- -UGCUCCAugAa--CCgCUCA-----------AGCGGCAG- -5' |
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16265 | 5' | -54.7 | NC_004084.1 | + | 2827 | 0.69 | 0.58107 |
Target: 5'- aACGAGGaACagcacgUUGGCGAGgaUGCCGa- -3' miRNA: 3'- -UGCUCCaUG------AACCGCUCaaGCGGCag -5' |
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16265 | 5' | -54.7 | NC_004084.1 | + | 36571 | 0.7 | 0.517714 |
Target: 5'- uCGAGGUACggucaguacuccgcaUGGCGAGUUCuCUGUg -3' miRNA: 3'- uGCUCCAUGa--------------ACCGCUCAAGcGGCAg -5' |
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16265 | 5' | -54.7 | NC_004084.1 | + | 28808 | 0.71 | 0.455307 |
Target: 5'- aGCcAGGUGCcguugugGGCGAgcaucgcgaccgGUUCGCCGUCu -3' miRNA: 3'- -UGcUCCAUGaa-----CCGCU------------CAAGCGGCAG- -5' |
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16265 | 5' | -54.7 | NC_004084.1 | + | 10238 | 0.71 | 0.455307 |
Target: 5'- uUGAGGUcGCcUGGUGAG-UCGCgGUCg -3' miRNA: 3'- uGCUCCA-UGaACCGCUCaAGCGgCAG- -5' |
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16265 | 5' | -54.7 | NC_004084.1 | + | 10895 | 0.72 | 0.416661 |
Target: 5'- gGCGAucgucgGGUACUcccgaucGGgGAGUUCGUCGUCu -3' miRNA: 3'- -UGCU------CCAUGAa------CCgCUCAAGCGGCAG- -5' |
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16265 | 5' | -54.7 | NC_004084.1 | + | 1240 | 0.77 | 0.202502 |
Target: 5'- uGCGAGGUgucguaucGCUUGGCGAGUUCG--GUCu -3' miRNA: 3'- -UGCUCCA--------UGAACCGCUCAAGCggCAG- -5' |
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16265 | 5' | -54.7 | NC_004084.1 | + | 49594 | 1.1 | 0.001035 |
Target: 5'- gACGAGGUACUUGGCGAGUUCGCCGUCg -3' miRNA: 3'- -UGCUCCAUGAACCGCUCAAGCGGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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