Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16268 | 3' | -54.6 | NC_004084.1 | + | 9016 | 0.66 | 0.805596 |
Target: 5'- uGACCACCgcguaGUCCUCGGaGAggGcauGGa -3' miRNA: 3'- -CUGGUGGag---UAGGAGCC-CUagCau-CC- -5' |
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16268 | 3' | -54.6 | NC_004084.1 | + | 53537 | 0.66 | 0.786369 |
Target: 5'- cGCCuCCUguUCUUCGGGAaCG-AGGu -3' miRNA: 3'- cUGGuGGAguAGGAGCCCUaGCaUCC- -5' |
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16268 | 3' | -54.6 | NC_004084.1 | + | 36885 | 0.66 | 0.776514 |
Target: 5'- gGGCCuCCUCAagCUCGGcGAacUCGgcGGc -3' miRNA: 3'- -CUGGuGGAGUagGAGCC-CU--AGCauCC- -5' |
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16268 | 3' | -54.6 | NC_004084.1 | + | 14493 | 0.66 | 0.766513 |
Target: 5'- cGGCUccuGCCUCGgccaaCUCGGGuUCGUGGc -3' miRNA: 3'- -CUGG---UGGAGUag---GAGCCCuAGCAUCc -5' |
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16268 | 3' | -54.6 | NC_004084.1 | + | 57262 | 0.66 | 0.766513 |
Target: 5'- cACCGCCUCcuucUCCggCGGGG-CGUucAGGu -3' miRNA: 3'- cUGGUGGAGu---AGGa-GCCCUaGCA--UCC- -5' |
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16268 | 3' | -54.6 | NC_004084.1 | + | 34931 | 0.67 | 0.756375 |
Target: 5'- cGGCCAUCUCGUCa-CGGGcguUCGUGc- -3' miRNA: 3'- -CUGGUGGAGUAGgaGCCCu--AGCAUcc -5' |
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16268 | 3' | -54.6 | NC_004084.1 | + | 49473 | 0.67 | 0.756375 |
Target: 5'- cGCCGCU--GUCCUCGGaGUCGUcGGc -3' miRNA: 3'- cUGGUGGagUAGGAGCCcUAGCAuCC- -5' |
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16268 | 3' | -54.6 | NC_004084.1 | + | 2826 | 0.67 | 0.746113 |
Target: 5'- uACgACCUCAaCCUCGgcguccgcgguGGAUCGcAGGa -3' miRNA: 3'- cUGgUGGAGUaGGAGC-----------CCUAGCaUCC- -5' |
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16268 | 3' | -54.6 | NC_004084.1 | + | 54937 | 0.67 | 0.746113 |
Target: 5'- cGAUCGggauCCUCcgCCUCGGGAU-GcGGGa -3' miRNA: 3'- -CUGGU----GGAGuaGGAGCCCUAgCaUCC- -5' |
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16268 | 3' | -54.6 | NC_004084.1 | + | 9230 | 0.67 | 0.732605 |
Target: 5'- uGACCugCUCgAUCagggcguccagcguCUCGGGGUCGUc-- -3' miRNA: 3'- -CUGGugGAG-UAG--------------GAGCCCUAGCAucc -5' |
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16268 | 3' | -54.6 | NC_004084.1 | + | 25258 | 0.67 | 0.72526 |
Target: 5'- aGAUCGCg--GUUCUCGGGAUCGUucuGGu -3' miRNA: 3'- -CUGGUGgagUAGGAGCCCUAGCAu--CC- -5' |
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16268 | 3' | -54.6 | NC_004084.1 | + | 5846 | 0.67 | 0.714691 |
Target: 5'- cGCCGCCgcaGUCCUCGuGGAUCu---- -3' miRNA: 3'- cUGGUGGag-UAGGAGC-CCUAGcaucc -5' |
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16268 | 3' | -54.6 | NC_004084.1 | + | 13915 | 0.68 | 0.682548 |
Target: 5'- cGACCGCUUCGcggaacUCCUCGucAUCGUuuAGGc -3' miRNA: 3'- -CUGGUGGAGU------AGGAGCccUAGCA--UCC- -5' |
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16268 | 3' | -54.6 | NC_004084.1 | + | 56464 | 0.68 | 0.671725 |
Target: 5'- cGAUCACgaUgAUCg-CGGGGUCGUAGGc -3' miRNA: 3'- -CUGGUGg-AgUAGgaGCCCUAGCAUCC- -5' |
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16268 | 3' | -54.6 | NC_004084.1 | + | 25538 | 0.68 | 0.660865 |
Target: 5'- -uCCACUagGUCCUCGGGAagGUAc- -3' miRNA: 3'- cuGGUGGagUAGGAGCCCUagCAUcc -5' |
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16268 | 3' | -54.6 | NC_004084.1 | + | 57105 | 0.69 | 0.632533 |
Target: 5'- aGACCGCCUCgauccagucgaggacGUCCUCGGuGUCGc--- -3' miRNA: 3'- -CUGGUGGAG---------------UAGGAGCCcUAGCaucc -5' |
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16268 | 3' | -54.6 | NC_004084.1 | + | 7273 | 0.69 | 0.595502 |
Target: 5'- cGAUCGCCUCcUCgUUCGGGAUCGc--- -3' miRNA: 3'- -CUGGUGGAGuAG-GAGCCCUAGCaucc -5' |
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16268 | 3' | -54.6 | NC_004084.1 | + | 24812 | 0.7 | 0.573861 |
Target: 5'- cGAUUACCUCAUCCUUaGGGA-CGgcGa -3' miRNA: 3'- -CUGGUGGAGUAGGAG-CCCUaGCauCc -5' |
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16268 | 3' | -54.6 | NC_004084.1 | + | 2359 | 0.7 | 0.573861 |
Target: 5'- -uCCGCgUCGUCCUCGaaGUCGUAGa -3' miRNA: 3'- cuGGUGgAGUAGGAGCccUAGCAUCc -5' |
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16268 | 3' | -54.6 | NC_004084.1 | + | 55707 | 0.73 | 0.36721 |
Target: 5'- cGACCGCCUCcaCCUCGaGAUCG-AGGc -3' miRNA: 3'- -CUGGUGGAGuaGGAGCcCUAGCaUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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