Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16268 | 5' | -53.7 | NC_004084.1 | + | 50005 | 0.66 | 0.840428 |
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Target: 5'- uCgCCCAUGAUCacguUGUCGccgaGCGGGGUGu -3' miRNA: 3'- -GgGGGUACUAGc---ACAGCc---UGCUCUAC- -5' |
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| 16268 | 5' | -53.7 | NC_004084.1 | + | 2520 | 0.67 | 0.813428 |
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Target: 5'- uCCUCCGgcgGAUCGUcGUCGcACG-GGUGg -3' miRNA: 3'- -GGGGGUa--CUAGCA-CAGCcUGCuCUAC- -5' |
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| 16268 | 5' | -53.7 | NC_004084.1 | + | 25 | 0.67 | 0.794477 |
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Target: 5'- --gUCGaGGUCGUGUCGGccacCGAGAUGg -3' miRNA: 3'- gggGGUaCUAGCACAGCCu---GCUCUAC- -5' |
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| 16268 | 5' | -53.7 | NC_004084.1 | + | 47664 | 0.68 | 0.73397 |
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Target: 5'- gCCCgAaGAUCG-GUCGGGCaGGGAUa -3' miRNA: 3'- gGGGgUaCUAGCaCAGCCUG-CUCUAc -5' |
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| 16268 | 5' | -53.7 | NC_004084.1 | + | 11100 | 0.7 | 0.626244 |
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Target: 5'- aCCUCCAUucGAUCGacgucucGUCGGACGAGu-- -3' miRNA: 3'- -GGGGGUA--CUAGCa------CAGCCUGCUCuac -5' |
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| 16268 | 5' | -53.7 | NC_004084.1 | + | 48771 | 1.09 | 0.001822 |
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Target: 5'- uCCCCCAUGAUCGUGUCGGACGAGAUGc -3' miRNA: 3'- -GGGGGUACUAGCACAGCCUGCUCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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