Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16269 | 3' | -59.6 | NC_004084.1 | + | 15762 | 0.66 | 0.499118 |
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Target: 5'- gGGAGGaCGUcGUCGGACuGcGCCGCc-- -3' miRNA: 3'- aCCUCC-GCGaCAGCUUG-C-CGGCGucg -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 14842 | 0.69 | 0.336516 |
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Target: 5'- cUGGAGGaucCGCgccucGUCGGgaugACGGCCGcCGGUc -3' miRNA: 3'- -ACCUCC---GCGa----CAGCU----UGCCGGC-GUCG- -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 10912 | 0.69 | 0.336516 |
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Target: 5'- aGGAGGCGCUGccacUCGAG-GGCCucgaGGUc -3' miRNA: 3'- aCCUCCGCGAC----AGCUUgCCGGcg--UCG- -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 10473 | 0.68 | 0.386276 |
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Target: 5'- aGGAcGGC-CUGaCGGACGGCCuCgAGCg -3' miRNA: 3'- aCCU-CCGcGACaGCUUGCCGGcG-UCG- -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 7360 | 0.66 | 0.499118 |
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Target: 5'- aGGAGGCGCgGuUCGcuccGACGGugaucCCGCcuGCg -3' miRNA: 3'- aCCUCCGCGaC-AGC----UUGCC-----GGCGu-CG- -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 4046 | 0.66 | 0.489122 |
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Target: 5'- aGGGGGCGgaGUUGA-CGGagaaGgGGCg -3' miRNA: 3'- aCCUCCGCgaCAGCUuGCCgg--CgUCG- -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 2723 | 0.67 | 0.440651 |
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Target: 5'- gUGGAGGCG--GUCGAACcagaGCgGCAGa -3' miRNA: 3'- -ACCUCCGCgaCAGCUUGc---CGgCGUCg -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 753 | 0.72 | 0.221662 |
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Target: 5'- cGGAGGCGCUGgaccaccaggcCGAuCGGCuCGUcgaGGCg -3' miRNA: 3'- aCCUCCGCGACa----------GCUuGCCG-GCG---UCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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