Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16269 | 3' | -59.6 | NC_004084.1 | + | 14842 | 0.69 | 0.336516 |
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Target: 5'- cUGGAGGaucCGCgccucGUCGGgaugACGGCCGcCGGUc -3' miRNA: 3'- -ACCUCC---GCGa----CAGCU----UGCCGGC-GUCG- -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 10912 | 0.69 | 0.336516 |
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Target: 5'- aGGAGGCGCUGccacUCGAG-GGCCucgaGGUc -3' miRNA: 3'- aCCUCCGCGAC----AGCUUgCCGGcg--UCG- -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 753 | 0.72 | 0.221662 |
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Target: 5'- cGGAGGCGCUGgaccaccaggcCGAuCGGCuCGUcgaGGCg -3' miRNA: 3'- aCCUCCGCGACa----------GCUuGCCG-GCG---UCG- -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 51157 | 0.72 | 0.210594 |
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Target: 5'- aGGAGGCGa--UCGAGaCGGUCGCcgAGCa -3' miRNA: 3'- aCCUCCGCgacAGCUU-GCCGGCG--UCG- -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 39834 | 0.73 | 0.180216 |
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Target: 5'- -uGAuGGCGCUcgaGUCcaucGGCGGCCGCGGCg -3' miRNA: 3'- acCU-CCGCGA---CAGc---UUGCCGGCGUCG- -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 22458 | 0.73 | 0.180216 |
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Target: 5'- cGGGGGCGCUcgCG-ACGGCUGCgugguGGCg -3' miRNA: 3'- aCCUCCGCGAcaGCuUGCCGGCG-----UCG- -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 17285 | 0.76 | 0.111226 |
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Target: 5'- gUGGAGGCGgUGgCG-GCGGUgGCGGCg -3' miRNA: 3'- -ACCUCCGCgACaGCuUGCCGgCGUCG- -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 48630 | 1.1 | 0.000337 |
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Target: 5'- gUGGAGGCGCUGUCGAACGGCCGCAGCc -3' miRNA: 3'- -ACCUCCGCGACAGCUUGCCGGCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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