Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16269 | 5' | -55.5 | NC_004084.1 | + | 3583 | 0.66 | 0.716003 |
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Target: 5'- cGAGCGGGCgaccgCGACGaacguCCUCCa- -3' miRNA: 3'- cUUCGCCCGaguaaGCUGU-----GGAGGca -5' |
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| 16269 | 5' | -55.5 | NC_004084.1 | + | 24254 | 0.67 | 0.672726 |
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Target: 5'- uGAAGCGGGC-CAUgucuUCGGgAUUUCCa- -3' miRNA: 3'- -CUUCGCCCGaGUA----AGCUgUGGAGGca -5' |
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| 16269 | 5' | -55.5 | NC_004084.1 | + | 56863 | 0.68 | 0.606864 |
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Target: 5'- --cGCGGGCUCcggcUCGACggccguGCCUCgGUc -3' miRNA: 3'- cuuCGCCCGAGua--AGCUG------UGGAGgCA- -5' |
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| 16269 | 5' | -55.5 | NC_004084.1 | + | 7096 | 0.69 | 0.520526 |
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Target: 5'- --cGCGGGCgCAcUCGAuCGCCUCgGUc -3' miRNA: 3'- cuuCGCCCGaGUaAGCU-GUGGAGgCA- -5' |
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| 16269 | 5' | -55.5 | NC_004084.1 | + | 28507 | 0.7 | 0.469023 |
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Target: 5'- uGgcGCGGGCUCGcaCGuuCACCUCUGc -3' miRNA: 3'- -CuuCGCCCGAGUaaGCu-GUGGAGGCa -5' |
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| 16269 | 5' | -55.5 | NC_004084.1 | + | 13776 | 0.71 | 0.420257 |
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Target: 5'- uGGGCGGGCUCGga-GAuCGCgUCCGg -3' miRNA: 3'- cUUCGCCCGAGUaagCU-GUGgAGGCa -5' |
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| 16269 | 5' | -55.5 | NC_004084.1 | + | 51914 | 0.71 | 0.401626 |
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Target: 5'- aGAGUGGGUUCAUccaguUCGACACgUUCGc -3' miRNA: 3'- cUUCGCCCGAGUA-----AGCUGUGgAGGCa -5' |
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| 16269 | 5' | -55.5 | NC_004084.1 | + | 50259 | 0.71 | 0.39251 |
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Target: 5'- uGGAGCGGGCUgG-UCGGCACgaCCa- -3' miRNA: 3'- -CUUCGCCCGAgUaAGCUGUGgaGGca -5' |
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| 16269 | 5' | -55.5 | NC_004084.1 | + | 48664 | 1.07 | 0.001263 |
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Target: 5'- gGAAGCGGGCUCAUUCGACACCUCCGUa -3' miRNA: 3'- -CUUCGCCCGAGUAAGCUGUGGAGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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