miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1627 5' -45.1 NC_001347.2 + 53047 0.66 1
Target:  5'- cGGUUUgGgcaacgcCCUGUCUGgcGUcUCCGUGg -3'
miRNA:   3'- -CCAAGgU-------GGAUAGACuuUAaAGGCAU- -5'
1627 5' -45.1 NC_001347.2 + 128285 0.66 1
Target:  5'- cGGcgCCACUgccgCUGcGAUUUCCGa- -3'
miRNA:   3'- -CCaaGGUGGaua-GACuUUAAAGGCau -5'
1627 5' -45.1 NC_001347.2 + 39702 0.71 0.999565
Target:  5'- uGGcgCCGCCUuggcgccacgcaGUCUGGAAgg-CCGUGg -3'
miRNA:   3'- -CCaaGGUGGA------------UAGACUUUaaaGGCAU- -5'
1627 5' -45.1 NC_001347.2 + 137290 1.1 0.04212
Target:  5'- gGGUUCCACCUAUCUGAAAUUUCCGUAc -3'
miRNA:   3'- -CCAAGGUGGAUAGACUUUAAAGGCAU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.