Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16270 | 3' | -54.7 | NC_004084.1 | + | 44342 | 0.69 | 0.614093 |
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Target: 5'- uCGACGUcuaCGUCGGUC---GCGGUGAcaACg -3' miRNA: 3'- -GCUGCA---GCAGCCGGucaUGCCACU--UG- -5' |
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| 16270 | 3' | -54.7 | NC_004084.1 | + | 45416 | 0.67 | 0.711329 |
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Target: 5'- uCGAgGUCGUCGaucgaGCCGGauguCGGUGcGACa -3' miRNA: 3'- -GCUgCAGCAGC-----CGGUCau--GCCAC-UUG- -5' |
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| 16270 | 3' | -54.7 | NC_004084.1 | + | 48121 | 0.71 | 0.476055 |
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Target: 5'- ---aGUUG-CGGCCGGUcgucgucACGGUGAGCa -3' miRNA: 3'- gcugCAGCaGCCGGUCA-------UGCCACUUG- -5' |
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| 16270 | 3' | -54.7 | NC_004084.1 | + | 48433 | 1.1 | 0.001192 |
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Target: 5'- uCGACGUCGUCGGCCAGUACGGUGAACa -3' miRNA: 3'- -GCUGCAGCAGCCGGUCAUGCCACUUG- -5' |
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| 16270 | 3' | -54.7 | NC_004084.1 | + | 53309 | 0.69 | 0.603237 |
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Target: 5'- uCGACG--GUCGGCCAGcugGCGGcccgccUGGGCa -3' miRNA: 3'- -GCUGCagCAGCCGGUCa--UGCC------ACUUG- -5' |
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| 16270 | 3' | -54.7 | NC_004084.1 | + | 54265 | 0.67 | 0.721895 |
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Target: 5'- gCGACGUCaUCGGCC-GUcUGGUaAGCa -3' miRNA: 3'- -GCUGCAGcAGCCGGuCAuGCCAcUUG- -5' |
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| 16270 | 3' | -54.7 | NC_004084.1 | + | 55563 | 0.66 | 0.802357 |
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Target: 5'- gCGACGaacUCGUCGGCCuGUuCGGc---- -3' miRNA: 3'- -GCUGC---AGCAGCCGGuCAuGCCacuug -5' |
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| 16270 | 3' | -54.7 | NC_004084.1 | + | 56604 | 0.71 | 0.477057 |
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Target: 5'- gCGGCGUCGUC-GCCGGU-CGGcGAGu -3' miRNA: 3'- -GCUGCAGCAGcCGGUCAuGCCaCUUg -5' |
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| 16270 | 3' | -54.7 | NC_004084.1 | + | 57411 | 0.76 | 0.279925 |
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Target: 5'- uCGACGUCGcCGGCgAGcucgaGGUGGACa -3' miRNA: 3'- -GCUGCAGCaGCCGgUCaug--CCACUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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