Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16270 | 3' | -54.7 | NC_004084.1 | + | 29892 | 0.7 | 0.538897 |
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Target: 5'- aGACGUCccCGGCCAcgcagaGGUGAACg -3' miRNA: 3'- gCUGCAGcaGCCGGUcaug--CCACUUG- -5' |
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| 16270 | 3' | -54.7 | NC_004084.1 | + | 56604 | 0.71 | 0.477057 |
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Target: 5'- gCGGCGUCGUC-GCCGGU-CGGcGAGu -3' miRNA: 3'- -GCUGCAGCAGcCGGUCAuGCCaCUUg -5' |
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| 16270 | 3' | -54.7 | NC_004084.1 | + | 48121 | 0.71 | 0.476055 |
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Target: 5'- ---aGUUG-CGGCCGGUcgucgucACGGUGAGCa -3' miRNA: 3'- gcugCAGCaGCCGGUCA-------UGCCACUUG- -5' |
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| 16270 | 3' | -54.7 | NC_004084.1 | + | 18709 | 0.72 | 0.457223 |
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Target: 5'- aCGGCGUCGUCgccgucacuGGCgGGUuCGGUcGAGCc -3' miRNA: 3'- -GCUGCAGCAG---------CCGgUCAuGCCA-CUUG- -5' |
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| 16270 | 3' | -54.7 | NC_004084.1 | + | 28073 | 0.73 | 0.39153 |
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Target: 5'- cCGACGcCGUCGGCCAccucgccgACGGUcAACg -3' miRNA: 3'- -GCUGCaGCAGCCGGUca------UGCCAcUUG- -5' |
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| 16270 | 3' | -54.7 | NC_004084.1 | + | 18812 | 0.74 | 0.324398 |
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Target: 5'- gGACGUCGcCGGCCAcGUccaggaggACGGUGGcGCg -3' miRNA: 3'- gCUGCAGCaGCCGGU-CA--------UGCCACU-UG- -5' |
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| 16270 | 3' | -54.7 | NC_004084.1 | + | 29589 | 0.75 | 0.286987 |
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Target: 5'- gGGCGUCGuUCGcGCgCAGUucgAUGGUGAGCg -3' miRNA: 3'- gCUGCAGC-AGC-CG-GUCA---UGCCACUUG- -5' |
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| 16270 | 3' | -54.7 | NC_004084.1 | + | 57411 | 0.76 | 0.279925 |
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Target: 5'- uCGACGUCGcCGGCgAGcucgaGGUGGACa -3' miRNA: 3'- -GCUGCAGCaGCCGgUCaug--CCACUUG- -5' |
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| 16270 | 3' | -54.7 | NC_004084.1 | + | 48433 | 1.1 | 0.001192 |
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Target: 5'- uCGACGUCGUCGGCCAGUACGGUGAACa -3' miRNA: 3'- -GCUGCAGCAGCCGGUCAUGCCACUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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