Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16273 | 5' | -51.7 | NC_004084.1 | + | 50957 | 0.69 | 0.776576 |
Target: 5'- -cGUCGcc-GCUCGA-GUCGCCGUCGg -3' miRNA: 3'- gcCAGCacuUGAGCUaUAGCGGCAGU- -5' |
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16273 | 5' | -51.7 | NC_004084.1 | + | 54112 | 0.68 | 0.851583 |
Target: 5'- aGGUC----ACUCGccagGUCGCCGUCGg -3' miRNA: 3'- gCCAGcacuUGAGCua--UAGCGGCAGU- -5' |
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16273 | 5' | -51.7 | NC_004084.1 | + | 54372 | 0.67 | 0.883794 |
Target: 5'- gCGGUCGgcaGAucACUCuGAcgcgAUCGCCGUUc -3' miRNA: 3'- -GCCAGCa--CU--UGAG-CUa---UAGCGGCAGu -5' |
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16273 | 5' | -51.7 | NC_004084.1 | + | 57120 | 0.74 | 0.516444 |
Target: 5'- -aGUCGaGGACguccUCGGUGUCGCCGUCu -3' miRNA: 3'- gcCAGCaCUUG----AGCUAUAGCGGCAGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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