Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16279 | 5' | -48.4 | NC_004084.1 | + | 35804 | 0.66 | 0.981071 |
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Target: 5'- gCC-GAUgcaguGUAcuCCGGGCCGAGCAc -3' miRNA: 3'- -GGaCUGaauuuCAU--GGCUCGGUUCGU- -5' |
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| 16279 | 5' | -48.4 | NC_004084.1 | + | 37552 | 0.66 | 0.977011 |
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Target: 5'- aCUGACcgUGGAGUggACCGAucaacucgucgacgaGCCGAGgCAc -3' miRNA: 3'- gGACUGa-AUUUCA--UGGCU---------------CGGUUC-GU- -5' |
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| 16279 | 5' | -48.4 | NC_004084.1 | + | 29146 | 0.67 | 0.949753 |
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Target: 5'- aCCUGGCgcugccgGgcGUACaaccugguuCGAGCCGGGCGc -3' miRNA: 3'- -GGACUGaa-----UuuCAUG---------GCUCGGUUCGU- -5' |
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| 16279 | 5' | -48.4 | NC_004084.1 | + | 1745 | 0.69 | 0.902381 |
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Target: 5'- aCCgaGACccgccgccGAAGUACCGAGCgGGGCc -3' miRNA: 3'- -GGa-CUGaa------UUUCAUGGCUCGgUUCGu -5' |
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| 16279 | 5' | -48.4 | NC_004084.1 | + | 10651 | 0.7 | 0.871543 |
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Target: 5'- uUCUGACagcgccGAGUGCCGuuugcgaucGCCGGGCAg -3' miRNA: 3'- -GGACUGaau---UUCAUGGCu--------CGGUUCGU- -5' |
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| 16279 | 5' | -48.4 | NC_004084.1 | + | 33066 | 0.76 | 0.539577 |
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Target: 5'- gCCUGA-UUGAAGUuCCGAGCCAcgAGCc -3' miRNA: 3'- -GGACUgAAUUUCAuGGCUCGGU--UCGu -5' |
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| 16279 | 5' | -48.4 | NC_004084.1 | + | 22810 | 0.79 | 0.422565 |
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Target: 5'- aCCUGACUgUGGAG-AUCGGGCCGGGUg -3' miRNA: 3'- -GGACUGA-AUUUCaUGGCUCGGUUCGu -5' |
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| 16279 | 5' | -48.4 | NC_004084.1 | + | 44624 | 1.11 | 0.003847 |
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Target: 5'- uCCUGACUUAAAGUACCGAGCCAAGCAc -3' miRNA: 3'- -GGACUGAAUUUCAUGGCUCGGUUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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