Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1628 | 3' | -53.7 | NC_001347.2 | + | 130127 | 0.66 | 0.981692 |
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Target: 5'- --uCUCGCgcguggcCGuCUCGCUACGcCGUCg -3' miRNA: 3'- aucGAGCGaa-----GC-GAGCGAUGCaGUAG- -5' |
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| 1628 | 3' | -53.7 | NC_001347.2 | + | 182942 | 0.67 | 0.966082 |
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Target: 5'- --aCUCGCcgCGCUCGCggGCGUCc-- -3' miRNA: 3'- aucGAGCGaaGCGAGCGa-UGCAGuag -5' |
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| 1628 | 3' | -53.7 | NC_001347.2 | + | 156212 | 0.67 | 0.966082 |
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Target: 5'- aAGCUCGUUUCGCcggCGCgccACGUa--- -3' miRNA: 3'- aUCGAGCGAAGCGa--GCGa--UGCAguag -5' |
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| 1628 | 3' | -53.7 | NC_001347.2 | + | 158335 | 0.69 | 0.917056 |
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Target: 5'- gAGCUCGUgcacUGCUCGCUcuGCGgcaGUCa -3' miRNA: 3'- aUCGAGCGaa--GCGAGCGA--UGCag-UAG- -5' |
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| 1628 | 3' | -53.7 | NC_001347.2 | + | 114196 | 0.7 | 0.878259 |
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Target: 5'- cAGC-CGCUgaGCUCGCUGCGUUu-- -3' miRNA: 3'- aUCGaGCGAagCGAGCGAUGCAGuag -5' |
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| 1628 | 3' | -53.7 | NC_001347.2 | + | 38417 | 0.72 | 0.814483 |
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Target: 5'- -cGCUCGC--CGCUgGCggcgACGUCGUCc -3' miRNA: 3'- auCGAGCGaaGCGAgCGa---UGCAGUAG- -5' |
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| 1628 | 3' | -53.7 | NC_001347.2 | + | 208120 | 0.75 | 0.639241 |
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Target: 5'- gAGC-CGCgUCGCUCGCcgGCGcUCGUCa -3' miRNA: 3'- aUCGaGCGaAGCGAGCGa-UGC-AGUAG- -5' |
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| 1628 | 3' | -53.7 | NC_001347.2 | + | 136969 | 0.92 | 0.071367 |
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Target: 5'- gUAGCUCGCUaCGCUCGCUACGUCAc- -3' miRNA: 3'- -AUCGAGCGAaGCGAGCGAUGCAGUag -5' |
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| 1628 | 3' | -53.7 | NC_001347.2 | + | 136902 | 1.09 | 0.006337 |
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Target: 5'- gUAGCUCGCUUCGCUCGCUACGUCAUCa -3' miRNA: 3'- -AUCGAGCGAAGCGAGCGAUGCAGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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