Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16280 | 5' | -61.1 | NC_004084.1 | + | 20823 | 0.66 | 0.41251 |
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Target: 5'- gUCuuGaGAGcGcUCCCCGAGAGUGCc- -3' miRNA: 3'- -AGggCgCUCaC-AGGGGCUCUCGCGuu -5' |
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| 16280 | 5' | -61.1 | NC_004084.1 | + | 47005 | 0.66 | 0.403542 |
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Target: 5'- -aCCGCGAGU-UCCUCGAGGacGuCGCGAu -3' miRNA: 3'- agGGCGCUCAcAGGGGCUCU--C-GCGUU- -5' |
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| 16280 | 5' | -61.1 | NC_004084.1 | + | 8066 | 0.66 | 0.394699 |
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Target: 5'- cUCCCGCGAGaaguUGUccaggagaaCCCCGAGGGagaggcccuUGCGGa -3' miRNA: 3'- -AGGGCGCUC----ACA---------GGGGCUCUC---------GCGUU- -5' |
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| 16280 | 5' | -61.1 | NC_004084.1 | + | 35406 | 0.67 | 0.344342 |
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Target: 5'- uUCCCGaGAGcGUUgUUGAGAGCGCGc -3' miRNA: 3'- -AGGGCgCUCaCAGgGGCUCUCGCGUu -5' |
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| 16280 | 5' | -61.1 | NC_004084.1 | + | 23298 | 0.69 | 0.2778 |
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Target: 5'- gUCCCgaGCGAGcgcGUgaCCGAGAGCGCGu -3' miRNA: 3'- -AGGG--CGCUCa--CAggGGCUCUCGCGUu -5' |
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| 16280 | 5' | -61.1 | NC_004084.1 | + | 57476 | 0.69 | 0.258017 |
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Target: 5'- gCCCGCGAcc-UCCuCCGGGAGCGUc- -3' miRNA: 3'- aGGGCGCUcacAGG-GGCUCUCGCGuu -5' |
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| 16280 | 5' | -61.1 | NC_004084.1 | + | 28911 | 0.72 | 0.162555 |
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Target: 5'- ---aGCGGGUGUgCCCGAGAGCGaCGu -3' miRNA: 3'- agggCGCUCACAgGGGCUCUCGC-GUu -5' |
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| 16280 | 5' | -61.1 | NC_004084.1 | + | 42966 | 1.06 | 0.000488 |
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Target: 5'- uUCCCGCGAGUGUCCCCGAGAGCGCAAc -3' miRNA: 3'- -AGGGCGCUCACAGGGGCUCUCGCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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