Results 61 - 64 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16283 | 5' | -53.5 | NC_004084.1 | + | 51519 | 0.67 | 0.774883 |
Target: 5'- -cUCgUcGaGACGGUCGCGAucCCGACGUa -3' miRNA: 3'- caAGgA-C-CUGCUAGCGCU--GGUUGCAg -5' |
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16283 | 5' | -53.5 | NC_004084.1 | + | 55370 | 0.67 | 0.77185 |
Target: 5'- aGUUCCUcGAgGGUgGCGAUCAucgccguauucgccACGUCg -3' miRNA: 3'- -CAAGGAcCUgCUAgCGCUGGU--------------UGCAG- -5' |
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16283 | 5' | -53.5 | NC_004084.1 | + | 57668 | 0.69 | 0.635353 |
Target: 5'- --gCCUGGugGuccaGCGccuCCGACGUCg -3' miRNA: 3'- caaGGACCugCuag-CGCu--GGUUGCAG- -5' |
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16283 | 5' | -53.5 | NC_004084.1 | + | 58242 | 0.72 | 0.493622 |
Target: 5'- -cUUCUGGgaccuucACGAUCGCGACCAcuACGcCu -3' miRNA: 3'- caAGGACC-------UGCUAGCGCUGGU--UGCaG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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