miRNA: 3'- caAGGa-CCugCUAGCG--CUGg--------UUGCAG- -5'
miRNA: 3'- cAAG-GAC----CUGCUAGC-GCUGG--------UUGCAG- -5'
miRNA: 3'- caAGgaCCUGCUAGCGCuGGUu--GcAG- -5'
miRNA: 3'- caaGGa-CCUGCuAGCGcUGGUUGCAG- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16283 | 5' | -53.5 | NC_004084.1 | + | 2844 | 0.66 | 0.823238 |
|
Target: 5'- -gUCCgcGGugGAUCGCagGACaccaucgaaGACGUCa -3' miRNA: 3'- caAGGa-CCugCUAGCG--CUGg--------UUGCAG- -5' |
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| 16283 | 5' | -53.5 | NC_004084.1 | + | 1467 | 0.67 | 0.774883 |
|
Target: 5'- cUUCgCUGaugcGGCGAUCGaCGACCuccugguaGACGUCg -3' miRNA: 3'- cAAG-GAC----CUGCUAGC-GCUGG--------UUGCAG- -5' |
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| 16283 | 5' | -53.5 | NC_004084.1 | + | 1237 | 0.66 | 0.804431 |
|
Target: 5'- -aUCgaGGGCGAUCGCGcCCAucuCcUCa -3' miRNA: 3'- caAGgaCCUGCUAGCGCuGGUu--GcAG- -5' |
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| 16283 | 5' | -53.5 | NC_004084.1 | + | 384 | 0.7 | 0.602213 |
|
Target: 5'- --cCCggGGACG-UCGCcGCCGAUGUCg -3' miRNA: 3'- caaGGa-CCUGCuAGCGcUGGUUGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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