Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16284 | 3' | -52.9 | NC_004084.1 | + | 52238 | 0.69 | 0.728245 |
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Target: 5'- --aUUCGAACucauCCGcCUUGCuGACGUCGc -3' miRNA: 3'- ccaGAGCUUGu---GGC-GAACG-CUGCAGU- -5' |
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| 16284 | 3' | -52.9 | NC_004084.1 | + | 12432 | 0.69 | 0.728245 |
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Target: 5'- cGUCUCGAACGCCGUga-CGAcCG-CAg -3' miRNA: 3'- cCAGAGCUUGUGGCGaacGCU-GCaGU- -5' |
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| 16284 | 3' | -52.9 | NC_004084.1 | + | 46690 | 0.7 | 0.663205 |
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Target: 5'- --aUUCGAGCACguCGCcgGCGACGUCu -3' miRNA: 3'- ccaGAGCUUGUG--GCGaaCGCUGCAGu -5' |
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| 16284 | 3' | -52.9 | NC_004084.1 | + | 12964 | 0.71 | 0.575192 |
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Target: 5'- cGGcUUCGAu--CCGCggGCGGCGUCAg -3' miRNA: 3'- -CCaGAGCUuguGGCGaaCGCUGCAGU- -5' |
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| 16284 | 3' | -52.9 | NC_004084.1 | + | 39592 | 0.72 | 0.553481 |
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Target: 5'- aGGUCcCGGGCG-CGCUUGUcuGGCGUCGc -3' miRNA: 3'- -CCAGaGCUUGUgGCGAACG--CUGCAGU- -5' |
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| 16284 | 3' | -52.9 | NC_004084.1 | + | 25463 | 0.72 | 0.542715 |
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Target: 5'- uGGcUUCGAGguUCGCggGCGGCGUCAg -3' miRNA: 3'- -CCaGAGCUUguGGCGaaCGCUGCAGU- -5' |
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| 16284 | 3' | -52.9 | NC_004084.1 | + | 1007 | 0.72 | 0.542715 |
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Target: 5'- --cCUCGAGC-UCGCcgGCGACGUCGa -3' miRNA: 3'- ccaGAGCUUGuGGCGaaCGCUGCAGU- -5' |
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| 16284 | 3' | -52.9 | NC_004084.1 | + | 30037 | 0.72 | 0.510867 |
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Target: 5'- aGGUCUCGcgGGgGCCGCUcGCGuCGUUg -3' miRNA: 3'- -CCAGAGC--UUgUGGCGAaCGCuGCAGu -5' |
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| 16284 | 3' | -52.9 | NC_004084.1 | + | 41503 | 0.73 | 0.479822 |
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Target: 5'- -aUCgCGGAUGCCGCUgGCGACGUUg -3' miRNA: 3'- ccAGaGCUUGUGGCGAaCGCUGCAGu -5' |
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| 16284 | 3' | -52.9 | NC_004084.1 | + | 41351 | 1.12 | 0.001395 |
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Target: 5'- cGGUCUCGAACACCGCUUGCGACGUCAg -3' miRNA: 3'- -CCAGAGCUUGUGGCGAACGCUGCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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