Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16286 | 3' | -54.6 | NC_004084.1 | + | 7399 | 0.72 | 0.437568 |
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Target: 5'- -cGACGAGCUGAgcaaggcgaUCGACgacgagggcGAUGCCGa -3' miRNA: 3'- ucCUGCUCGACU---------AGCUGa--------CUACGGCg -5' |
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| 16286 | 3' | -54.6 | NC_004084.1 | + | 8379 | 0.72 | 0.408166 |
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Target: 5'- aGGGACGAGCaucuUCGACUuucgucgGAUGuuGCu -3' miRNA: 3'- -UCCUGCUCGacu-AGCUGA-------CUACggCG- -5' |
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| 16286 | 3' | -54.6 | NC_004084.1 | + | 38489 | 0.72 | 0.399864 |
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Target: 5'- uGGACGAGCUGGUCGA--GAUcgGCCu- -3' miRNA: 3'- uCCUGCUCGACUAGCUgaCUA--CGGcg -5' |
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| 16286 | 3' | -54.6 | NC_004084.1 | + | 47019 | 0.73 | 0.390767 |
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Target: 5'- gAGGACGucGC-GAUCGAUgacGAUGCCGg -3' miRNA: 3'- -UCCUGCu-CGaCUAGCUGa--CUACGGCg -5' |
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| 16286 | 3' | -54.6 | NC_004084.1 | + | 55980 | 0.74 | 0.310562 |
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Target: 5'- cGGAgGGGCUGAUCGccgauggcgagcaucGCUGGcgaGCCGCc -3' miRNA: 3'- uCCUgCUCGACUAGC---------------UGACUa--CGGCG- -5' |
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| 16286 | 3' | -54.6 | NC_004084.1 | + | 17678 | 0.76 | 0.242881 |
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Target: 5'- cAGGGCGAcGCUGGUCGAgcuucgagacgcuaCUGA-GCCGUu -3' miRNA: 3'- -UCCUGCU-CGACUAGCU--------------GACUaCGGCG- -5' |
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| 16286 | 3' | -54.6 | NC_004084.1 | + | 22440 | 0.8 | 0.138745 |
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Target: 5'- cGGaGACGAGCUGAUCGAgucgggaCUGGUcacgGCCGCc -3' miRNA: 3'- -UC-CUGCUCGACUAGCU-------GACUA----CGGCG- -5' |
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| 16286 | 3' | -54.6 | NC_004084.1 | + | 40695 | 1.13 | 0.000709 |
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Target: 5'- gAGGACGAGCUGAUCGACUGAUGCCGCg -3' miRNA: 3'- -UCCUGCUCGACUAGCUGACUACGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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