Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16294 | 5' | -48.7 | NC_004084.1 | + | 56910 | 0.67 | 0.956904 |
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Target: 5'- gUGCAUCGGcuuCGAGCU-CGAgggCGa -3' miRNA: 3'- aACGUAGCCacuGCUUGAaGCUaa-GC- -5' |
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| 16294 | 5' | -48.7 | NC_004084.1 | + | 56921 | 0.69 | 0.899685 |
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Target: 5'- --uCGUCGGgacGGCGGACUUCGuagUCGu -3' miRNA: 3'- aacGUAGCCa--CUGCUUGAAGCua-AGC- -5' |
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| 16294 | 5' | -48.7 | NC_004084.1 | + | 52216 | 0.7 | 0.868519 |
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Target: 5'- -gGCGUCGGUcucGCGAGCcUCGAU-CGa -3' miRNA: 3'- aaCGUAGCCAc--UGCUUGaAGCUAaGC- -5' |
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| 16294 | 5' | -48.7 | NC_004084.1 | + | 10812 | 0.71 | 0.813919 |
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Target: 5'- aUGuCAUCGGUGACGAGa-UCGAcaCGa -3' miRNA: 3'- aAC-GUAGCCACUGCUUgaAGCUaaGC- -5' |
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| 16294 | 5' | -48.7 | NC_004084.1 | + | 22434 | 0.72 | 0.783658 |
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Target: 5'- -cGCgGUCGGaGACGAGCUgaUCGAgUCGg -3' miRNA: 3'- aaCG-UAGCCaCUGCUUGA--AGCUaAGC- -5' |
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| 16294 | 5' | -48.7 | NC_004084.1 | + | 36845 | 1.08 | 0.006 |
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Target: 5'- gUUGCAUCGGUGACGAACUUCGAUUCGa -3' miRNA: 3'- -AACGUAGCCACUGCUUGAAGCUAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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