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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 16295 | 3' | -53.7 | NC_004084.1 | + | 29402 | 0.74 | 0.422947 |
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Target: 5'- gGCGGccacuccuggGGCGUCGCGGuugC-CGUCGACg -3' miRNA: 3'- -CGCUa---------CUGCAGCGCCua-GaGCAGCUG- -5' |
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| 16295 | 3' | -53.7 | NC_004084.1 | + | 39280 | 0.74 | 0.386372 |
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Target: 5'- uCGAUcGCGUCGCGGAcUCgaUCGUCGAg -3' miRNA: 3'- cGCUAcUGCAGCGCCU-AG--AGCAGCUg -5' |
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| 16295 | 3' | -53.7 | NC_004084.1 | + | 4164 | 0.74 | 0.386372 |
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Target: 5'- cCGAUGACGUCGCGGuugCUCGg---- -3' miRNA: 3'- cGCUACUGCAGCGCCua-GAGCagcug -5' |
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| 16295 | 3' | -53.7 | NC_004084.1 | + | 632 | 0.74 | 0.413609 |
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Target: 5'- gGCGAUcuCGUCGUGGAgCUCcUCGACg -3' miRNA: 3'- -CGCUAcuGCAGCGCCUaGAGcAGCUG- -5' |
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| 16295 | 3' | -53.7 | NC_004084.1 | + | 18833 | 0.74 | 0.413609 |
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Target: 5'- -gGAgGACGguggCGCGGcUUUCGUCGACg -3' miRNA: 3'- cgCUaCUGCa---GCGCCuAGAGCAGCUG- -5' |
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| 16295 | 3' | -53.7 | NC_004084.1 | + | 50907 | 0.75 | 0.360345 |
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Target: 5'- uGCGAUGGCccagcaGCGGAUCggccucgaCGUCGACg -3' miRNA: 3'- -CGCUACUGcag---CGCCUAGa-------GCAGCUG- -5' |
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| 16295 | 3' | -53.7 | NC_004084.1 | + | 19649 | 0.75 | 0.360345 |
|
Target: 5'- aCGAUGGCGUCcauGUGGAgccagaacugUUCGUCGACg -3' miRNA: 3'- cGCUACUGCAG---CGCCUa---------GAGCAGCUG- -5' |
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| 16295 | 3' | -53.7 | NC_004084.1 | + | 46414 | 0.78 | 0.242794 |
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Target: 5'- gGCGAaguuguUGACGUCGCuGGGgaaCUCGUCGAg -3' miRNA: 3'- -CGCU------ACUGCAGCG-CCUa--GAGCAGCUg -5' |
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| 16295 | 3' | -53.7 | NC_004084.1 | + | 55958 | 0.81 | 0.146481 |
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Target: 5'- cGCGAUccaGCGUgGCucGGAUCUCGUCGACg -3' miRNA: 3'- -CGCUAc--UGCAgCG--CCUAGAGCAGCUG- -5' |
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| 16295 | 3' | -53.7 | NC_004084.1 | + | 36697 | 1.12 | 0.001157 |
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Target: 5'- uGCGAUGACGUCGCGGAUCUCGUCGACg -3' miRNA: 3'- -CGCUACUGCAGCGCCUAGAGCAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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