Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16295 | 5' | -53.5 | NC_004084.1 | + | 15724 | 0.68 | 0.680521 |
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Target: 5'- uCGUCAGCaucgGUGaUCGCug-GCAGCCu -3' miRNA: 3'- uGCAGUCGa---CACgAGCGuugCGUUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 37726 | 0.68 | 0.668346 |
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Target: 5'- cCGUUAGCUucggccuccuggaGUaGCUCGUAcgcGCGCGAUCg -3' miRNA: 3'- uGCAGUCGA-------------CA-CGAGCGU---UGCGUUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 42101 | 0.7 | 0.58052 |
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Target: 5'- -gGUCAGUgcugcgGUGUUCGCAGugguCGCuGCCg -3' miRNA: 3'- ugCAGUCGa-----CACGAGCGUU----GCGuUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 6340 | 0.71 | 0.536797 |
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Target: 5'- aACGUCGGCgaUGUaCGCGacgaagggGCGCGACCu -3' miRNA: 3'- -UGCAGUCGacACGaGCGU--------UGCGUUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 42260 | 0.72 | 0.473588 |
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Target: 5'- -gGUCgAGUgaUGCUCgaGCAGCGCGACCa -3' miRNA: 3'- ugCAG-UCGacACGAG--CGUUGCGUUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 18557 | 0.72 | 0.453349 |
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Target: 5'- aGC-UCAGC-GUGCUCGaCGGCGCcgcGGCCg -3' miRNA: 3'- -UGcAGUCGaCACGAGC-GUUGCG---UUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 46472 | 0.73 | 0.422937 |
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Target: 5'- uACGUCAGCUcgaucgaguccucGcUGgUCGCGACGUcGCCg -3' miRNA: 3'- -UGCAGUCGA-------------C-ACgAGCGUUGCGuUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 47884 | 0.78 | 0.221864 |
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Target: 5'- uCGUCcGCUG-GCUCgGCAACGCGACg -3' miRNA: 3'- uGCAGuCGACaCGAG-CGUUGCGUUGg -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 36731 | 1.12 | 0.00098 |
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Target: 5'- gACGUCAGCUGUGCUCGCAACGCAACCu -3' miRNA: 3'- -UGCAGUCGACACGAGCGUUGCGUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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