Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16295 | 5' | -53.5 | NC_004084.1 | + | 41183 | 0.66 | 0.82145 |
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Target: 5'- -gGUCAGCUGggacgaacacgacGCcCGCGACGCuggcgaaacGCCg -3' miRNA: 3'- ugCAGUCGACa------------CGaGCGUUGCGu--------UGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 42101 | 0.7 | 0.58052 |
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Target: 5'- -gGUCAGUgcugcgGUGUUCGCAGugguCGCuGCCg -3' miRNA: 3'- ugCAGUCGa-----CACGAGCGUU----GCGuUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 42260 | 0.72 | 0.473588 |
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Target: 5'- -gGUCgAGUgaUGCUCgaGCAGCGCGACCa -3' miRNA: 3'- ugCAG-UCGacACGAG--CGUUGCGUUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 44054 | 0.67 | 0.756012 |
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Target: 5'- cCGUaCAGaugGUGaucCUCGCcACGCGGCCa -3' miRNA: 3'- uGCA-GUCga-CAC---GAGCGuUGCGUUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 46472 | 0.73 | 0.422937 |
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Target: 5'- uACGUCAGCUcgaucgaguccucGcUGgUCGCGACGUcGCCg -3' miRNA: 3'- -UGCAGUCGA-------------C-ACgAGCGUUGCGuUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 47884 | 0.78 | 0.221864 |
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Target: 5'- uCGUCcGCUG-GCUCgGCAACGCGACg -3' miRNA: 3'- uGCAGuCGACaCGAG-CGUUGCGUUGg -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 52850 | 0.67 | 0.776592 |
|
Target: 5'- aGCGgCAGUUGacgaucUGCUCG-AGCGguGCCg -3' miRNA: 3'- -UGCaGUCGAC------ACGAGCgUUGCguUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 53579 | 0.66 | 0.796558 |
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Target: 5'- gUGcCGGaUGUGCUCGCGACGauCGGCg -3' miRNA: 3'- uGCaGUCgACACGAGCGUUGC--GUUGg -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 55024 | 0.68 | 0.713391 |
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Target: 5'- uGCGUCucGUUGUGCUCGaggaGCuccGCCa -3' miRNA: 3'- -UGCAGu-CGACACGAGCguugCGu--UGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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