Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16295 | 5' | -53.5 | NC_004084.1 | + | 18557 | 0.72 | 0.453349 |
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Target: 5'- aGC-UCAGC-GUGCUCGaCGGCGCcgcGGCCg -3' miRNA: 3'- -UGcAGUCGaCACGAGC-GUUGCG---UUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 16888 | 0.66 | 0.78265 |
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Target: 5'- gGCGUCugcuccgaucgacGCUGUGa--GCAACGUcGCCa -3' miRNA: 3'- -UGCAGu------------CGACACgagCGUUGCGuUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 15724 | 0.68 | 0.680521 |
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Target: 5'- uCGUCAGCaucgGUGaUCGCug-GCAGCCu -3' miRNA: 3'- uGCAGUCGa---CACgAGCGuugCGUUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 7244 | 0.66 | 0.825161 |
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Target: 5'- gACGaUCGGCgcGUGCUCG----GCGACCg -3' miRNA: 3'- -UGC-AGUCGa-CACGAGCguugCGUUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 6340 | 0.71 | 0.536797 |
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Target: 5'- aACGUCGGCgaUGUaCGCGacgaagggGCGCGACCu -3' miRNA: 3'- -UGCAGUCGacACGaGCGU--------UGCGUUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 4525 | 0.66 | 0.796558 |
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Target: 5'- uCG-CAGCgcGUGCagGCGAUGCAggGCCu -3' miRNA: 3'- uGCaGUCGa-CACGagCGUUGCGU--UGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 2525 | 0.66 | 0.825161 |
|
Target: 5'- uCGUCuGCaUGUuCUUGCAGaGCAGCCc -3' miRNA: 3'- uGCAGuCG-ACAcGAGCGUUgCGUUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 1836 | 0.67 | 0.756012 |
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Target: 5'- cGCGuUCAcGgUGUgGCUCcuGCAGCGCGACg -3' miRNA: 3'- -UGC-AGU-CgACA-CGAG--CGUUGCGUUGg -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 1213 | 0.67 | 0.734913 |
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Target: 5'- aACGcUGGCUG-GCUCGgCGGCGgGAUCg -3' miRNA: 3'- -UGCaGUCGACaCGAGC-GUUGCgUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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