Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16295 | 5' | -53.5 | NC_004084.1 | + | 29144 | 0.68 | 0.69154 |
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Target: 5'- gUGUCGGCgGUGCUgGUGuggucGCGCgGGCCg -3' miRNA: 3'- uGCAGUCGaCACGAgCGU-----UGCG-UUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 1213 | 0.67 | 0.734913 |
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Target: 5'- aACGcUGGCUG-GCUCGgCGGCGgGAUCg -3' miRNA: 3'- -UGCaGUCGACaCGAGC-GUUGCgUUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 1836 | 0.67 | 0.756012 |
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Target: 5'- cGCGuUCAcGgUGUgGCUCcuGCAGCGCGACg -3' miRNA: 3'- -UGC-AGU-CgACA-CGAG--CGUUGCGUUGg -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 28982 | 0.67 | 0.776592 |
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Target: 5'- cGCGUUcGCgGUgaaGCUCGCcgucGACGgCAACCg -3' miRNA: 3'- -UGCAGuCGaCA---CGAGCG----UUGC-GUUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 16888 | 0.66 | 0.78265 |
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Target: 5'- gGCGUCugcuccgaucgacGCUGUGa--GCAACGUcGCCa -3' miRNA: 3'- -UGCAGu------------CGACACgagCGUUGCGuUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 29667 | 0.66 | 0.786657 |
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Target: 5'- cGCGccggUAGCUccGgggGUUCGCGACGCggUCg -3' miRNA: 3'- -UGCa---GUCGA--Ca--CGAGCGUUGCGuuGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 4525 | 0.66 | 0.796558 |
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Target: 5'- uCG-CAGCgcGUGCagGCGAUGCAggGCCu -3' miRNA: 3'- uGCaGUCGa-CACGagCGUUGCGU--UGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 35546 | 0.66 | 0.806283 |
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Target: 5'- gACcUCGGCggcgaGUGC-CGCcgGACGCAGCUc -3' miRNA: 3'- -UGcAGUCGa----CACGaGCG--UUGCGUUGG- -5' |
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| 16295 | 5' | -53.5 | NC_004084.1 | + | 47884 | 0.78 | 0.221864 |
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Target: 5'- uCGUCcGCUG-GCUCgGCAACGCGACg -3' miRNA: 3'- uGCAGuCGACaCGAG-CGUUGCGUUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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