Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16296 | 3' | -51.2 | NC_004084.1 | + | 2891 | 0.71 | 0.741311 |
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Target: 5'- gCGAUCGCGGaggaucccaucauCAacGCGGUGcCGACGAgGa -3' miRNA: 3'- -GCUAGCGCU-------------GU--UGCCAUaGCUGCUgC- -5' |
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| 16296 | 3' | -51.2 | NC_004084.1 | + | 2429 | 0.7 | 0.763049 |
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Target: 5'- gCGAUCGUGG--GCGGUgaAUCGAccguCGACGa -3' miRNA: 3'- -GCUAGCGCUguUGCCA--UAGCU----GCUGC- -5' |
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| 16296 | 3' | -51.2 | NC_004084.1 | + | 1973 | 0.7 | 0.784194 |
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Target: 5'- uGAUCGagGACAACGGgaugcagacuacgucGUCGAgGACGc -3' miRNA: 3'- gCUAGCg-CUGUUGCCa--------------UAGCUgCUGC- -5' |
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| 16296 | 3' | -51.2 | NC_004084.1 | + | 1720 | 0.67 | 0.921427 |
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Target: 5'- uGGUCGCGGCGAgucgagcgUGGcGUaGAUGACGa -3' miRNA: 3'- gCUAGCGCUGUU--------GCCaUAgCUGCUGC- -5' |
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| 16296 | 3' | -51.2 | NC_004084.1 | + | 848 | 0.67 | 0.908248 |
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Target: 5'- ---gCGUGACAugGGUguggaaagagucaGUCGuCGACGu -3' miRNA: 3'- gcuaGCGCUGUugCCA-------------UAGCuGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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