miRNA: 3'- gcUAGC-GCUGU---UGCCauAGCUGCUGC- -5'
miRNA: 3'- -GCU---AGcGCUGU---UGCCaUAGCUGCUGc -5'
miRNA: 3'- -GCUAGCGCUGUu---GCcaUAGCUGCuGC- -5'
miRNA: 3'- -GCUAGCGCUGU---UGCcAUAGCUGCUgc -5'
miRNA: 3'- -GCU--AGC-GCU-GUUGC-CAU-AGCUGCUGc -5'
Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16296 | 3' | -51.2 | NC_004084.1 | + | 55254 | 0.67 | 0.90223 |
|
Target: 5'- -cGUCGaCGACGucuACGG--UCGGCGGCGu -3' miRNA: 3'- gcUAGC-GCUGU---UGCCauAGCUGCUGC- -5' |
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| 16296 | 3' | -51.2 | NC_004084.1 | + | 18348 | 0.67 | 0.90223 |
|
Target: 5'- uCGAccaUCcCGACGuucGCGGgGUCGACGGCu -3' miRNA: 3'- -GCU---AGcGCUGU---UGCCaUAGCUGCUGc -5' |
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| 16296 | 3' | -51.2 | NC_004084.1 | + | 16631 | 0.68 | 0.888077 |
|
Target: 5'- uCGAUCGUGACGcccuCGucGUCGACGuCGu -3' miRNA: 3'- -GCUAGCGCUGUu---GCcaUAGCUGCuGC- -5' |
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| 16296 | 3' | -51.2 | NC_004084.1 | + | 26120 | 0.68 | 0.880607 |
|
Target: 5'- cCGGUCGCGACGaucGCGuUGUCGGUGAg- -3' miRNA: 3'- -GCUAGCGCUGU---UGCcAUAGCUGCUgc -5' |
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| 16296 | 3' | -51.2 | NC_004084.1 | + | 7236 | 0.66 | 0.947903 |
|
Target: 5'- cCGAccUCGaCGAuCGGCGcGUGcUCGGCGACc -3' miRNA: 3'- -GCU--AGC-GCU-GUUGC-CAU-AGCUGCUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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