Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16297 | 5' | -56 | NC_004084.1 | + | 19505 | 0.66 | 0.727442 |
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Target: 5'- aGGAGCCcGGGAGCGaa--CG-CCGGUu -3' miRNA: 3'- -CCUCGGcUCUUCGCacuaGCaGGCCA- -5' |
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| 16297 | 5' | -56 | NC_004084.1 | + | 53167 | 0.66 | 0.727442 |
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Target: 5'- -aGGCCGAuGAcugaccccAGCGUGuUCGUCCGu- -3' miRNA: 3'- ccUCGGCU-CU--------UCGCACuAGCAGGCca -5' |
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| 16297 | 5' | -56 | NC_004084.1 | + | 25246 | 0.66 | 0.706452 |
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Target: 5'- aGGAGCucguCGAGAucGCGguucucggGAUCGuUCUGGUa -3' miRNA: 3'- -CCUCG----GCUCUu-CGCa-------CUAGC-AGGCCA- -5' |
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| 16297 | 5' | -56 | NC_004084.1 | + | 29717 | 0.67 | 0.674469 |
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Target: 5'- -cGGCCGuucGAGGa--GAUCGUCCGGg -3' miRNA: 3'- ccUCGGCu--CUUCgcaCUAGCAGGCCa -5' |
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| 16297 | 5' | -56 | NC_004084.1 | + | 11319 | 0.67 | 0.663716 |
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Target: 5'- aGGuGCCGAccGGCG-GGUUGUCgGGa -3' miRNA: 3'- -CCuCGGCUcuUCGCaCUAGCAGgCCa -5' |
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| 16297 | 5' | -56 | NC_004084.1 | + | 48310 | 0.67 | 0.642137 |
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Target: 5'- cGAGUCGAGcagugcAGUGUGAUCGUCgCGc- -3' miRNA: 3'- cCUCGGCUCu-----UCGCACUAGCAG-GCca -5' |
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| 16297 | 5' | -56 | NC_004084.1 | + | 40688 | 0.69 | 0.556183 |
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Target: 5'- cGGAGCCGAGGacgAGC-UGAUCGaCUGa- -3' miRNA: 3'- -CCUCGGCUCU---UCGcACUAGCaGGCca -5' |
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| 16297 | 5' | -56 | NC_004084.1 | + | 45368 | 0.69 | 0.535123 |
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Target: 5'- gGGAaCCGAaucgucAGCGUgucGAUCGUCCGGUc -3' miRNA: 3'- -CCUcGGCUcu----UCGCA---CUAGCAGGCCA- -5' |
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| 16297 | 5' | -56 | NC_004084.1 | + | 50121 | 0.74 | 0.285697 |
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Target: 5'- cGAGUCGAGGccCGcGAUCGUCCGGa -3' miRNA: 3'- cCUCGGCUCUucGCaCUAGCAGGCCa -5' |
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| 16297 | 5' | -56 | NC_004084.1 | + | 36012 | 1.1 | 0.000864 |
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Target: 5'- cGGAGCCGAGAAGCGUGAUCGUCCGGUa -3' miRNA: 3'- -CCUCGGCUCUUCGCACUAGCAGGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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