Results 41 - 47 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16298 | 3' | -55.2 | NC_004084.1 | + | 17328 | 0.67 | 0.738104 |
Target: 5'- cCGCGG-CGACGGcccgCAUCGucUCGUCGa -3' miRNA: 3'- aGUGCUgGCUGCCca--GUAGC--AGCAGC- -5' |
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16298 | 3' | -55.2 | NC_004084.1 | + | 49665 | 0.67 | 0.727831 |
Target: 5'- aC-CGGCUGAagcCGGGUgGUCcGUCGUCu -3' miRNA: 3'- aGuGCUGGCU---GCCCAgUAG-CAGCAGc -5' |
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16298 | 3' | -55.2 | NC_004084.1 | + | 18696 | 0.67 | 0.707024 |
Target: 5'- gUCAuccuCG-UCGACGGcGUCGUCGcCGUCa -3' miRNA: 3'- -AGU----GCuGGCUGCC-CAGUAGCaGCAGc -5' |
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16298 | 3' | -55.2 | NC_004084.1 | + | 45852 | 0.68 | 0.696511 |
Target: 5'- aUCGCuugcCCGucuucggugaACGGGaCGUCGUCGUCGc -3' miRNA: 3'- -AGUGcu--GGC----------UGCCCaGUAGCAGCAGC- -5' |
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16298 | 3' | -55.2 | NC_004084.1 | + | 50132 | 0.68 | 0.696511 |
Target: 5'- cCGCGAUCguccggauGACGaGGUCGUUGUCGagGa -3' miRNA: 3'- aGUGCUGG--------CUGC-CCAGUAGCAGCagC- -5' |
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16298 | 3' | -55.2 | NC_004084.1 | + | 18976 | 0.68 | 0.685939 |
Target: 5'- cCAguCGGCCGGCGGGgaaGUCGUagGUCc -3' miRNA: 3'- aGU--GCUGGCUGCCCag-UAGCAg-CAGc -5' |
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16298 | 3' | -55.2 | NC_004084.1 | + | 26190 | 0.66 | 0.806589 |
Target: 5'- -aACGACCGuCGGcGUCGUCGagGa-- -3' miRNA: 3'- agUGCUGGCuGCC-CAGUAGCagCagc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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