Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16301 | 5' | -53.8 | NC_004084.1 | + | 3104 | 0.66 | 0.807571 |
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Target: 5'- gCGGGCUCCUcauccacGAGUaCGggGCUGGGAu- -3' miRNA: 3'- -GCUCGAGGAc------UUCA-GCa-UGACCCUcg -5' |
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| 16301 | 5' | -53.8 | NC_004084.1 | + | 51988 | 0.66 | 0.797961 |
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Target: 5'- uGGGCguucgaaCUGAAGcCGcgggACUGGGAGa -3' miRNA: 3'- gCUCGag-----GACUUCaGCa---UGACCCUCg -5' |
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| 16301 | 5' | -53.8 | NC_004084.1 | + | 36212 | 0.67 | 0.768131 |
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Target: 5'- uCGAGCgUCgUGAcGUUGUGCUcGGAGa -3' miRNA: 3'- -GCUCG-AGgACUuCAGCAUGAcCCUCg -5' |
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| 16301 | 5' | -53.8 | NC_004084.1 | + | 1581 | 0.67 | 0.737033 |
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Target: 5'- aCGGG-UCCaGGAcgacGUCGcccgGCUGGGAGCu -3' miRNA: 3'- -GCUCgAGGaCUU----CAGCa---UGACCCUCG- -5' |
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| 16301 | 5' | -53.8 | NC_004084.1 | + | 13754 | 0.68 | 0.715751 |
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Target: 5'- -cGGCUcgcCCUcGAGGUCGcGCUGGGcgGGCu -3' miRNA: 3'- gcUCGA---GGA-CUUCAGCaUGACCC--UCG- -5' |
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| 16301 | 5' | -53.8 | NC_004084.1 | + | 30103 | 0.68 | 0.704979 |
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Target: 5'- uGuAGCUCCgGAcGUCGcug-GGGAGCg -3' miRNA: 3'- gC-UCGAGGaCUuCAGCaugaCCCUCG- -5' |
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| 16301 | 5' | -53.8 | NC_004084.1 | + | 42613 | 0.69 | 0.650271 |
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Target: 5'- cCGAcGUUCCUcGgcGUUGUcaacgACUGGGAGUu -3' miRNA: 3'- -GCU-CGAGGA-CuuCAGCA-----UGACCCUCG- -5' |
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| 16301 | 5' | -53.8 | NC_004084.1 | + | 54017 | 0.7 | 0.584161 |
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Target: 5'- aGcAGCUCgCcGAAGUCGUGCUGcuuGGGCu -3' miRNA: 3'- gC-UCGAG-GaCUUCAGCAUGACc--CUCG- -5' |
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| 16301 | 5' | -53.8 | NC_004084.1 | + | 33186 | 1.12 | 0.001024 |
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Target: 5'- aCGAGCUCCUGAAGUCGUACUGGGAGCc -3' miRNA: 3'- -GCUCGAGGACUUCAGCAUGACCCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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