Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16303 | 5' | -53.8 | NC_004084.1 | + | 23753 | 0.77 | 0.240435 |
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Target: 5'- aAUGGACGCGGUGAGcgAC-UCCcAGACg -3' miRNA: 3'- -UACCUGCGCCAUUCa-UGcAGGcUCUG- -5' |
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| 16303 | 5' | -53.8 | NC_004084.1 | + | 23783 | 0.77 | 0.240435 |
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Target: 5'- aAUGGACGCGGUGAGcgAC-UCCcAGACg -3' miRNA: 3'- -UACCUGCGCCAUUCa-UGcAGGcUCUG- -5' |
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| 16303 | 5' | -53.8 | NC_004084.1 | + | 23813 | 0.76 | 0.27162 |
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Target: 5'- aAUGGACGCGGUGA--GCGUCUccaccgucgcgaugGAGGCa -3' miRNA: 3'- -UACCUGCGCCAUUcaUGCAGG--------------CUCUG- -5' |
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| 16303 | 5' | -53.8 | NC_004084.1 | + | 23693 | 0.76 | 0.28803 |
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Target: 5'- aAUGGACGCGGUGA--GCGUCUcccAGACg -3' miRNA: 3'- -UACCUGCGCCAUUcaUGCAGGc--UCUG- -5' |
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| 16303 | 5' | -53.8 | NC_004084.1 | + | 24055 | 0.7 | 0.546637 |
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Target: 5'- gAUGGACGCGGUugcgGCGUCCa---- -3' miRNA: 3'- -UACCUGCGCCAuucaUGCAGGcucug -5' |
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| 16303 | 5' | -53.8 | NC_004084.1 | + | 39609 | 0.7 | 0.590406 |
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Target: 5'- cUGGACGUGGccGGcgACGUCCucGACg -3' miRNA: 3'- uACCUGCGCCauUCa-UGCAGGcuCUG- -5' |
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| 16303 | 5' | -53.8 | NC_004084.1 | + | 24025 | 0.7 | 0.590406 |
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Target: 5'- gAUGGACGCGGU---UGCGggcUCCcAGACg -3' miRNA: 3'- -UACCUGCGCCAuucAUGC---AGGcUCUG- -5' |
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| 16303 | 5' | -53.8 | NC_004084.1 | + | 23965 | 0.97 | 0.010188 |
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Target: 5'- gAUGGACGCGGUAAGUACGUCCGAaACa -3' miRNA: 3'- -UACCUGCGCCAUUCAUGCAGGCUcUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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