Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16304 | 3' | -56.6 | NC_004084.1 | + | 41033 | 0.66 | 0.633407 |
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Target: 5'- aCUCuGCCGGCgaGaUCCucacCGGCGGAAc -3' miRNA: 3'- -GAGcCGGUCGaaC-AGGuu--GUCGCCUU- -5' |
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| 16304 | 3' | -56.6 | NC_004084.1 | + | 12225 | 0.66 | 0.611287 |
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Target: 5'- aCUCGGCCAGCUcGUCgAGgAGUu--- -3' miRNA: 3'- -GAGCCGGUCGAaCAGgUUgUCGccuu -5' |
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| 16304 | 3' | -56.6 | NC_004084.1 | + | 56807 | 0.66 | 0.58924 |
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Target: 5'- aUCGGCCAucuucgaccGCUUGUcugCCAACcagcGCGGGc -3' miRNA: 3'- gAGCCGGU---------CGAACA---GGUUGu---CGCCUu -5' |
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| 16304 | 3' | -56.6 | NC_004084.1 | + | 52706 | 0.67 | 0.562981 |
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Target: 5'- cCUCGGCCcgAGCgacGUCCucgaacucaaccCAGCGGAc -3' miRNA: 3'- -GAGCCGG--UCGaa-CAGGuu----------GUCGCCUu -5' |
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| 16304 | 3' | -56.6 | NC_004084.1 | + | 5267 | 0.67 | 0.556463 |
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Target: 5'- aUCGGCCucGUcgacgacGUCCcGCAGCGGAu -3' miRNA: 3'- gAGCCGGu-CGaa-----CAGGuUGUCGCCUu -5' |
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| 16304 | 3' | -56.6 | NC_004084.1 | + | 44190 | 0.68 | 0.510485 |
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Target: 5'- -cCGGCCAGUaUGUCUcgguugaggaccucGACgAGCGGAu -3' miRNA: 3'- gaGCCGGUCGaACAGG--------------UUG-UCGCCUu -5' |
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| 16304 | 3' | -56.6 | NC_004084.1 | + | 43786 | 0.69 | 0.404146 |
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Target: 5'- gCUC-GCCGGCggagaGUCCGGCGGCGaGGAg -3' miRNA: 3'- -GAGcCGGUCGaa---CAGGUUGUCGC-CUU- -5' |
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| 16304 | 3' | -56.6 | NC_004084.1 | + | 18979 | 0.7 | 0.398602 |
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Target: 5'- gUCGGCCGGCggggaagucguagGUCCcGCuGGCGGGGu -3' miRNA: 3'- gAGCCGGUCGaa-----------CAGGuUG-UCGCCUU- -5' |
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| 16304 | 3' | -56.6 | NC_004084.1 | + | 31016 | 1.07 | 0.000935 |
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Target: 5'- aCUCGGCCAGCUUGUCCAACAGCGGAAg -3' miRNA: 3'- -GAGCCGGUCGAACAGGUUGUCGCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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