Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 16306 | 5' | -49.6 | NC_004084.1 | + | 29579 | 0.75 | 0.562785 |
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Target: 5'- aUCGCCGGGugGGCG-UCGUucGCGc -3' miRNA: 3'- gAGUGGUCUugCCGCuAGCAuuUGCu -5' |
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| 16306 | 5' | -49.6 | NC_004084.1 | + | 48185 | 0.75 | 0.573933 |
|
Target: 5'- aCUCACCAGGcgaccucaaGCGGCGA-CGgguGGCGGa -3' miRNA: 3'- -GAGUGGUCU---------UGCCGCUaGCau-UUGCU- -5' |
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| 16306 | 5' | -49.6 | NC_004084.1 | + | 11576 | 0.76 | 0.540674 |
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Target: 5'- uUCACgGacGCGGCGAUCGUGGGCa- -3' miRNA: 3'- gAGUGgUcuUGCCGCUAGCAUUUGcu -5' |
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| 16306 | 5' | -49.6 | NC_004084.1 | + | 51306 | 0.76 | 0.529727 |
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Target: 5'- aUCACCAaGACGGCGAcCG-AGGCGAu -3' miRNA: 3'- gAGUGGUcUUGCCGCUaGCaUUUGCU- -5' |
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| 16306 | 5' | -49.6 | NC_004084.1 | + | 21472 | 0.77 | 0.452716 |
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Target: 5'- gCUCGCCcucgAGGGCGGCGAucgccucgguugcgUCGUAGGCGu -3' miRNA: 3'- -GAGUGG----UCUUGCCGCU--------------AGCAUUUGCu -5' |
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| 16306 | 5' | -49.6 | NC_004084.1 | + | 29943 | 1.1 | 0.003924 |
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Target: 5'- gCUCACCAGAACGGCGAUCGUAAACGAa -3' miRNA: 3'- -GAGUGGUCUUGCCGCUAGCAUUUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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