Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16306 | 5' | -49.6 | NC_004084.1 | + | 1179 | 0.68 | 0.918092 |
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Target: 5'- aUCGCCG--AC-GCGGUCGUGGACGu -3' miRNA: 3'- gAGUGGUcuUGcCGCUAGCAUUUGCu -5' |
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| 16306 | 5' | -49.6 | NC_004084.1 | + | 278 | 0.68 | 0.918092 |
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Target: 5'- --aGCUGGGgcagACGGUGAUCGUGucGCGAa -3' miRNA: 3'- gagUGGUCU----UGCCGCUAGCAUu-UGCU- -5' |
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| 16306 | 5' | -49.6 | NC_004084.1 | + | 21991 | 0.68 | 0.907641 |
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Target: 5'- gUCguaCAGGucuGCGGCGAUCGUcgaaagaccgugaccGAGCGAg -3' miRNA: 3'- gAGug-GUCU---UGCCGCUAGCA---------------UUUGCU- -5' |
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| 16306 | 5' | -49.6 | NC_004084.1 | + | 1454 | 0.68 | 0.904223 |
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Target: 5'- uUCGCuCGGAccgcuucgcugauGCGGCGAUCG---ACGAc -3' miRNA: 3'- gAGUG-GUCU-------------UGCCGCUAGCauuUGCU- -5' |
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| 16306 | 5' | -49.6 | NC_004084.1 | + | 56612 | 0.68 | 0.897892 |
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Target: 5'- gUCGCCGGu-CGGCGAguucgccggaUCGUggcucaGAACGAc -3' miRNA: 3'- gAGUGGUCuuGCCGCU----------AGCA------UUUGCU- -5' |
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| 16306 | 5' | -49.6 | NC_004084.1 | + | 29445 | 0.66 | 0.970072 |
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Target: 5'- uUCACCGcGAAC-GCGG-CGUGGGCGc -3' miRNA: 3'- gAGUGGU-CUUGcCGCUaGCAUUUGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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