Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16309 | 5' | -58.1 | NC_004084.1 | + | 30578 | 0.71 | 0.285398 |
Target: 5'- cGACgGUCGGCGaaCCgGAGCGGGGUGAGg- -3' miRNA: 3'- -UUG-UAGCUGC--GG-CUCGUCCCGCUCaa -5' |
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16309 | 5' | -58.1 | NC_004084.1 | + | 29221 | 0.73 | 0.209429 |
Target: 5'- cGCGUCGACGuuGuucGC-GGGCGAGUa -3' miRNA: 3'- uUGUAGCUGCggCu--CGuCCCGCUCAa -5' |
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16309 | 5' | -58.1 | NC_004084.1 | + | 50016 | 0.73 | 0.188274 |
Target: 5'- cACGUUGuCGCCGAGCGGGGUGucccGGUc -3' miRNA: 3'- uUGUAGCuGCGGCUCGUCCCGC----UCAa -5' |
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16309 | 5' | -58.1 | NC_004084.1 | + | 41641 | 0.74 | 0.178418 |
Target: 5'- gGACAUCGACGUCGAGUucaaacgccGGGCGAa-- -3' miRNA: 3'- -UUGUAGCUGCGGCUCGu--------CCCGCUcaa -5' |
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16309 | 5' | -58.1 | NC_004084.1 | + | 29226 | 1.02 | 0.001354 |
Target: 5'- gAACAUCGACGCCGAGCAGGGCGAGUUc -3' miRNA: 3'- -UUGUAGCUGCGGCUCGUCCCGCUCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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