Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16310 | 3' | -57.1 | NC_004084.1 | + | 6144 | 0.66 | 0.676745 |
Target: 5'- gCGACCAGCGCcgGGGUUCcu-GGCGa- -3' miRNA: 3'- aGCUGGUCGCG--CUCAAGcucUCGCgc -5' |
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16310 | 3' | -57.1 | NC_004084.1 | + | 34552 | 0.66 | 0.676745 |
Target: 5'- gCGAUCAcCGCGuccaucgCGAGGGCGCu -3' miRNA: 3'- aGCUGGUcGCGCucaa---GCUCUCGCGc -5' |
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16310 | 3' | -57.1 | NC_004084.1 | + | 43754 | 0.66 | 0.676745 |
Target: 5'- cCGGCCGGgacgcuCGCGGGcaUCGAcuGGGCGCu -3' miRNA: 3'- aGCUGGUC------GCGCUCa-AGCU--CUCGCGc -5' |
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16310 | 3' | -57.1 | NC_004084.1 | + | 25254 | 0.66 | 0.676745 |
Target: 5'- aCGGCCuccuuGCGCGGGagCGAGuGCa-- -3' miRNA: 3'- aGCUGGu----CGCGCUCaaGCUCuCGcgc -5' |
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16310 | 3' | -57.1 | NC_004084.1 | + | 40746 | 0.66 | 0.666053 |
Target: 5'- cUCGACCAGCGuCGcccuGUgcUCGGGuGuCGCc -3' miRNA: 3'- -AGCUGGUCGC-GCu---CA--AGCUCuC-GCGc -5' |
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16310 | 3' | -57.1 | NC_004084.1 | + | 27307 | 0.66 | 0.659623 |
Target: 5'- cUCGACgAGC-UGAuauucgaccguucacGUUCGAGGGaCGCGg -3' miRNA: 3'- -AGCUGgUCGcGCU---------------CAAGCUCUC-GCGC- -5' |
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16310 | 3' | -57.1 | NC_004084.1 | + | 38671 | 0.66 | 0.65533 |
Target: 5'- -aGACCuGCgGCGAGgucagCGAGaAGUGCu -3' miRNA: 3'- agCUGGuCG-CGCUCaa---GCUC-UCGCGc -5' |
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16310 | 3' | -57.1 | NC_004084.1 | + | 9830 | 0.66 | 0.644587 |
Target: 5'- cUCGAuCCAGCacuaCGAGgUCGGcGAGgGCGg -3' miRNA: 3'- -AGCU-GGUCGc---GCUCaAGCU-CUCgCGC- -5' |
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16310 | 3' | -57.1 | NC_004084.1 | + | 39458 | 0.66 | 0.644587 |
Target: 5'- cCGACUGGCGCGAGcgccgaUCGAGgAG-GCc -3' miRNA: 3'- aGCUGGUCGCGCUCa-----AGCUC-UCgCGc -5' |
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16310 | 3' | -57.1 | NC_004084.1 | + | 41324 | 0.66 | 0.623078 |
Target: 5'- cCGGCgAGCGCGGGUgagaaUGGGAGU-CGg -3' miRNA: 3'- aGCUGgUCGCGCUCAa----GCUCUCGcGC- -5' |
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16310 | 3' | -57.1 | NC_004084.1 | + | 29237 | 0.66 | 0.623078 |
Target: 5'- cCGAgCAGgGCGAGUUCcgcuGGAcgaacggcccGCGCGa -3' miRNA: 3'- aGCUgGUCgCGCUCAAGc---UCU----------CGCGC- -5' |
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16310 | 3' | -57.1 | NC_004084.1 | + | 51980 | 0.67 | 0.61233 |
Target: 5'- gCGACCAGUGgGcGUUCGAacugaAGcCGCGg -3' miRNA: 3'- aGCUGGUCGCgCuCAAGCUc----UC-GCGC- -5' |
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16310 | 3' | -57.1 | NC_004084.1 | + | 15424 | 0.67 | 0.601598 |
Target: 5'- cUCGAUC-GCGuCGAGUuccucaUCGAG-GCGCu -3' miRNA: 3'- -AGCUGGuCGC-GCUCA------AGCUCuCGCGc -5' |
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16310 | 3' | -57.1 | NC_004084.1 | + | 35400 | 0.67 | 0.601598 |
Target: 5'- aCGACCuucccgagAGCGUug--UUGAGAGCGCGc -3' miRNA: 3'- aGCUGG--------UCGCGcucaAGCUCUCGCGC- -5' |
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16310 | 3' | -57.1 | NC_004084.1 | + | 22424 | 0.67 | 0.595171 |
Target: 5'- aUUGGCCGGUcuaCGAGgccaauaccgccgucUUCGGGGGCGCu -3' miRNA: 3'- -AGCUGGUCGc--GCUC---------------AAGCUCUCGCGc -5' |
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16310 | 3' | -57.1 | NC_004084.1 | + | 29191 | 0.67 | 0.590892 |
Target: 5'- cUCGcccuGCuCGGCGuCGAuGUUCcAGAGCGCGu -3' miRNA: 3'- -AGC----UG-GUCGC-GCU-CAAGcUCUCGCGC- -5' |
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16310 | 3' | -57.1 | NC_004084.1 | + | 18467 | 0.67 | 0.569586 |
Target: 5'- aUGACCcGCuCGAGUUCGAG-GUGuCGa -3' miRNA: 3'- aGCUGGuCGcGCUCAAGCUCuCGC-GC- -5' |
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16310 | 3' | -57.1 | NC_004084.1 | + | 6547 | 0.68 | 0.548475 |
Target: 5'- cUCGACCGGCGUccgguAGUUCGAcucgucGAGCcacGCGc -3' miRNA: 3'- -AGCUGGUCGCGc----UCAAGCU------CUCG---CGC- -5' |
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16310 | 3' | -57.1 | NC_004084.1 | + | 37580 | 0.68 | 0.53801 |
Target: 5'- gUCGACgAGC-CGAGgcacucUCGGGgAGCGCu -3' miRNA: 3'- -AGCUGgUCGcGCUCa-----AGCUC-UCGCGc -5' |
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16310 | 3' | -57.1 | NC_004084.1 | + | 56889 | 0.68 | 0.531764 |
Target: 5'- cUCGGuCCAGaccgacCGCGAGUgcaucggcuucgagcUCGAGGGCGaCGu -3' miRNA: 3'- -AGCU-GGUC------GCGCUCA---------------AGCUCUCGC-GC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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