Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16311 | 3' | -57.4 | NC_004084.1 | + | 42709 | 0.67 | 0.575555 |
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Target: 5'- aUGCUGa-CGACCCUGuCGCGucGGCGAGu -3' miRNA: 3'- cAUGGCaaGUUGGGGC-GCGU--CCGCUC- -5' |
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| 16311 | 3' | -57.4 | NC_004084.1 | + | 15799 | 0.67 | 0.569146 |
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Target: 5'- -gACCGUUCGgacgccuuuacccugGCCCUGaacgacaGCAGGCuuGAGg -3' miRNA: 3'- caUGGCAAGU---------------UGGGGCg------CGUCCG--CUC- -5' |
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| 16311 | 3' | -57.4 | NC_004084.1 | + | 40061 | 0.67 | 0.543706 |
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Target: 5'- -aGCCG-UCGACCCCGCGaacGuCGGGa -3' miRNA: 3'- caUGGCaAGUUGGGGCGCgucC-GCUC- -5' |
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| 16311 | 3' | -57.4 | NC_004084.1 | + | 47922 | 0.68 | 0.481968 |
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Target: 5'- gGUGCCGcgCGAUCCaGgGCAauGGCGAGc -3' miRNA: 3'- -CAUGGCaaGUUGGGgCgCGU--CCGCUC- -5' |
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| 16311 | 3' | -57.4 | NC_004084.1 | + | 28011 | 0.68 | 0.46018 |
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Target: 5'- -gGCCGggguauucgucgUCGAaaagCCCGUGCAGGCGGu -3' miRNA: 3'- caUGGCa-----------AGUUg---GGGCGCGUCCGCUc -5' |
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| 16311 | 3' | -57.4 | NC_004084.1 | + | 29985 | 0.7 | 0.405393 |
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Target: 5'- uGUACCGgcgUCGACCUgGuCGCuGGUGAc -3' miRNA: 3'- -CAUGGCa--AGUUGGGgC-GCGuCCGCUc -5' |
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| 16311 | 3' | -57.4 | NC_004084.1 | + | 32316 | 0.72 | 0.291379 |
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Target: 5'- -gACCGggaacgUCcuGAUCCCaGCGCAGGCGAa -3' miRNA: 3'- caUGGCa-----AG--UUGGGG-CGCGUCCGCUc -5' |
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| 16311 | 3' | -57.4 | NC_004084.1 | + | 3366 | 0.73 | 0.263773 |
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Target: 5'- -cACCGUUCAACCCgGUGaagauGGCGGa -3' miRNA: 3'- caUGGCAAGUUGGGgCGCgu---CCGCUc -5' |
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| 16311 | 3' | -57.4 | NC_004084.1 | + | 28790 | 0.95 | 0.007506 |
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Target: 5'- ---aCGUUCAACCCCGCGCAGGCGAGc -3' miRNA: 3'- caugGCAAGUUGGGGCGCGUCCGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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