Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16314 | 3' | -59.3 | NC_004084.1 | + | 54217 | 0.66 | 0.499543 |
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Target: 5'- cAGCgGCUCgaguCGGAUCUCGAcGUCGGAg- -3' miRNA: 3'- -UUGaCGAG----GCCUAGGGCU-CGGCCUag -5' |
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| 16314 | 3' | -59.3 | NC_004084.1 | + | 40289 | 0.66 | 0.479417 |
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Target: 5'- gGACUccaUCCGGG--CCGAGCCGGAc- -3' miRNA: 3'- -UUGAcg-AGGCCUagGGCUCGGCCUag -5' |
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| 16314 | 3' | -59.3 | NC_004084.1 | + | 14666 | 0.67 | 0.440416 |
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Target: 5'- --aUGC-CCGcGAgcgUCCCG-GCCGGGUCc -3' miRNA: 3'- uugACGaGGC-CU---AGGGCuCGGCCUAG- -5' |
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| 16314 | 3' | -59.3 | NC_004084.1 | + | 48250 | 0.67 | 0.43095 |
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Target: 5'- gGACUGggaUUCCGaGAaCCuCGAGCCGGAg- -3' miRNA: 3'- -UUGAC---GAGGC-CUaGG-GCUCGGCCUag -5' |
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| 16314 | 3' | -59.3 | NC_004084.1 | + | 16721 | 0.69 | 0.343288 |
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Target: 5'- gAACUcaUCgGGAUCCUGuacgaacucGGCCGGAUCg -3' miRNA: 3'- -UUGAcgAGgCCUAGGGC---------UCGGCCUAG- -5' |
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| 16314 | 3' | -59.3 | NC_004084.1 | + | 27090 | 1.06 | 0.000645 |
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Target: 5'- gAACUGCUCCGGAUCCCGAGCCGGAUCc -3' miRNA: 3'- -UUGACGAGGCCUAGGGCUCGGCCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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