Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16316 | 3' | -56.4 | NC_004084.1 | + | 1497 | 0.68 | 0.603506 |
Target: 5'- -gGUAGAcGUcgcCCUCGAGCUCGaAGCCg -3' miRNA: 3'- agCAUCUcCA---GGAGCUUGGGC-UCGGg -5' |
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16316 | 3' | -56.4 | NC_004084.1 | + | 1119 | 0.67 | 0.657203 |
Target: 5'- cUCGUcgaGGAGGUCCUgGAugGCgcgguuguagaCGAGCUCg -3' miRNA: 3'- -AGCA---UCUCCAGGAgCU--UGg----------GCUCGGG- -5' |
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16316 | 3' | -56.4 | NC_004084.1 | + | 815 | 0.72 | 0.351292 |
Target: 5'- aUCGUGGAGGUCCUCG---UCGAuGCUg -3' miRNA: 3'- -AGCAUCUCCAGGAGCuugGGCU-CGGg -5' |
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16316 | 3' | -56.4 | NC_004084.1 | + | 345 | 0.67 | 0.64647 |
Target: 5'- gUCGUugAGccAGGUggccUCUCGAaacuguGCCgCGAGCCCg -3' miRNA: 3'- -AGCA--UC--UCCA----GGAGCU------UGG-GCUCGGG- -5' |
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16316 | 3' | -56.4 | NC_004084.1 | + | 8 | 0.68 | 0.571497 |
Target: 5'- -aGUcGGGGGUCUUCGAAgUCGAGgUCg -3' miRNA: 3'- agCA-UCUCCAGGAGCUUgGGCUCgGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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