Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16320 | 5' | -58.7 | NC_004084.1 | + | 3204 | 0.66 | 0.553271 |
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Target: 5'- gGCGGUGGCGaagCUCACCacugGAGGUGa- -3' miRNA: 3'- -UGUCACUGCggaGAGUGG----CUCCGCgu -5' |
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| 16320 | 5' | -58.7 | NC_004084.1 | + | 56217 | 0.66 | 0.553271 |
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Target: 5'- gGC-GUGugGCCgugcaUCUUGCCGucccGGCGCu -3' miRNA: 3'- -UGuCACugCGG-----AGAGUGGCu---CCGCGu -5' |
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| 16320 | 5' | -58.7 | NC_004084.1 | + | 20380 | 0.66 | 0.511632 |
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Target: 5'- cGCAGgauuuuucgagGAUGCCUCU-GgUGAGGCGCu -3' miRNA: 3'- -UGUCa----------CUGCGGAGAgUgGCUCCGCGu -5' |
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| 16320 | 5' | -58.7 | NC_004084.1 | + | 36919 | 0.67 | 0.481273 |
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Target: 5'- uGCGGUGA-GCCUacgacgcaACCGAGGCGa- -3' miRNA: 3'- -UGUCACUgCGGAgag-----UGGCUCCGCgu -5' |
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| 16320 | 5' | -58.7 | NC_004084.1 | + | 23819 | 0.67 | 0.442218 |
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Target: 5'- cGCGGUGAgCGUCUC-CACCGucGCGa- -3' miRNA: 3'- -UGUCACU-GCGGAGaGUGGCucCGCgu -5' |
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| 16320 | 5' | -58.7 | NC_004084.1 | + | 30302 | 0.69 | 0.353069 |
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Target: 5'- uCAuUGACGCg-CUCGUCGAGGCGCAc -3' miRNA: 3'- uGUcACUGCGgaGAGUGGCUCCGCGU- -5' |
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| 16320 | 5' | -58.7 | NC_004084.1 | + | 11736 | 0.69 | 0.344883 |
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Target: 5'- aGCAGUGGCGCgUCauggUCcUCGAGGCGa- -3' miRNA: 3'- -UGUCACUGCGgAG----AGuGGCUCCGCgu -5' |
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| 16320 | 5' | -58.7 | NC_004084.1 | + | 47593 | 0.7 | 0.312755 |
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Target: 5'- cGCGGUGGCGaCUCUCACaagaucuCGAGGUGg- -3' miRNA: 3'- -UGUCACUGCgGAGAGUG-------GCUCCGCgu -5' |
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| 16320 | 5' | -58.7 | NC_004084.1 | + | 36251 | 0.76 | 0.126195 |
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Target: 5'- -gGGUcGACGuCCUCgagugCGCCGAGGCGCu -3' miRNA: 3'- ugUCA-CUGC-GGAGa----GUGGCUCCGCGu -5' |
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| 16320 | 5' | -58.7 | NC_004084.1 | + | 23745 | 1.08 | 0.000563 |
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Target: 5'- cACAGUGACGCCUCUCACCGAGGCGCAa -3' miRNA: 3'- -UGUCACUGCGGAGAGUGGCUCCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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