Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16329 | 5' | -57.1 | NC_004084.1 | + | 56618 | 0.66 | 0.652412 |
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Target: 5'- gGUcGGCGAGuUCGCCGGAucgUGGCuCAg -3' miRNA: 3'- -CAcUUGCUCcAGCGGCCUca-ACCG-GU- -5' |
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| 16329 | 5' | -57.1 | NC_004084.1 | + | 40218 | 0.66 | 0.641566 |
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Target: 5'- cUGGACGAGGagaCGCgGaAGcUGGCCAu -3' miRNA: 3'- cACUUGCUCCa--GCGgCcUCaACCGGU- -5' |
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| 16329 | 5' | -57.1 | NC_004084.1 | + | 55897 | 0.66 | 0.641566 |
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Target: 5'- cGUGcGACGAcGaucCGCCGGAGgagugGGCCGa -3' miRNA: 3'- -CAC-UUGCUcCa--GCGGCCUCaa---CCGGU- -5' |
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| 16329 | 5' | -57.1 | NC_004084.1 | + | 35928 | 0.67 | 0.544664 |
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Target: 5'- cGUucACGAGcauguccccggcGUCGCCGGGGUcggcGGCCGu -3' miRNA: 3'- -CAcuUGCUC------------CAGCGGCCUCAa---CCGGU- -5' |
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| 16329 | 5' | -57.1 | NC_004084.1 | + | 53553 | 0.69 | 0.472659 |
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Target: 5'- -gGAACGAGGUCGUCcugcuuguGGAGgu-GCCGg -3' miRNA: 3'- caCUUGCUCCAGCGG--------CCUCaacCGGU- -5' |
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| 16329 | 5' | -57.1 | NC_004084.1 | + | 36087 | 0.7 | 0.396711 |
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Target: 5'- --cGACGAGGUCGCCGucuccuacGAGUggcacGGCCc -3' miRNA: 3'- cacUUGCUCCAGCGGC--------CUCAa----CCGGu -5' |
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| 16329 | 5' | -57.1 | NC_004084.1 | + | 18618 | 0.72 | 0.291324 |
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Target: 5'- gGUGGGCucGAaGUCGCC-GAGUUGGCCGa -3' miRNA: 3'- -CACUUG--CUcCAGCGGcCUCAACCGGU- -5' |
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| 16329 | 5' | -57.1 | NC_004084.1 | + | 18229 | 1.08 | 0.000862 |
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Target: 5'- gGUGAACGAGGUCGCCGGAGUUGGCCAu -3' miRNA: 3'- -CACUUGCUCCAGCGGCCUCAACCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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