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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 16332 | 5' | -52.9 | NC_004084.1 | + | 55376 | 0.71 | 0.636563 |
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Target: 5'- uCGAGGGugGCGAUCAUCGccGUAUuCGCCa -3' miRNA: 3'- -GCUCUC--UGCUGGUAGCccUAUA-GCGG- -5' |
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| 16332 | 5' | -52.9 | NC_004084.1 | + | 41084 | 0.72 | 0.528253 |
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Target: 5'- uCGAcGAGACGAUg--CGGGccGUCGCCg -3' miRNA: 3'- -GCU-CUCUGCUGguaGCCCuaUAGCGG- -5' |
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| 16332 | 5' | -52.9 | NC_004084.1 | + | 42312 | 0.72 | 0.560262 |
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Target: 5'- gCGAcGAucgcGACGACCGUCGGGAcgagAUCGaCg -3' miRNA: 3'- -GCU-CU----CUGCUGGUAGCCCUa---UAGCgG- -5' |
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| 16332 | 5' | -52.9 | NC_004084.1 | + | 28922 | 0.72 | 0.581899 |
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Target: 5'- cCGAGAGcgacguCGGCC-UCGGGAgcgCGUCg -3' miRNA: 3'- -GCUCUCu-----GCUGGuAGCCCUauaGCGG- -5' |
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| 16332 | 5' | -52.9 | NC_004084.1 | + | 34726 | 0.73 | 0.52193 |
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Target: 5'- uGGGAGACGcucaccgcguccauuGCCGUCuGGGA-GUCGCUc -3' miRNA: 3'- gCUCUCUGC---------------UGGUAG-CCCUaUAGCGG- -5' |
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| 16332 | 5' | -52.9 | NC_004084.1 | + | 31675 | 0.73 | 0.507294 |
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Target: 5'- ----cGAUGGCCGUCGGGAaguUCGCUg -3' miRNA: 3'- gcucuCUGCUGGUAGCCCUau-AGCGG- -5' |
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| 16332 | 5' | -52.9 | NC_004084.1 | + | 37862 | 0.73 | 0.507294 |
|
Target: 5'- uCGAacGGGAUGGCCGggCGGGugucggAUCGCCu -3' miRNA: 3'- -GCU--CUCUGCUGGUa-GCCCua----UAGCGG- -5' |
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| 16332 | 5' | -52.9 | NC_004084.1 | + | 21075 | 0.75 | 0.399504 |
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Target: 5'- uCGAGcuGGGCG-UCGUCGGGA--UCGCCg -3' miRNA: 3'- -GCUC--UCUGCuGGUAGCCCUauAGCGG- -5' |
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| 16332 | 5' | -52.9 | NC_004084.1 | + | 205 | 0.75 | 0.399504 |
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Target: 5'- cCGGGAGcccgcACGGCCAUCGGcGGgggaGUCGUCg -3' miRNA: 3'- -GCUCUC-----UGCUGGUAGCC-CUa---UAGCGG- -5' |
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| 16332 | 5' | -52.9 | NC_004084.1 | + | 16482 | 1.13 | 0.001239 |
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Target: 5'- uCGAGAGACGACCAUCGGGAUAUCGCCa -3' miRNA: 3'- -GCUCUCUGCUGGUAGCCCUAUAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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