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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16332 | 5' | -52.9 | NC_004084.1 | + | 42312 | 0.72 | 0.560262 |
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Target: 5'- gCGAcGAucgcGACGACCGUCGGGAcgagAUCGaCg -3' miRNA: 3'- -GCU-CU----CUGCUGGUAGCCCUa---UAGCgG- -5' |
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| 16332 | 5' | -52.9 | NC_004084.1 | + | 44220 | 0.67 | 0.840694 |
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Target: 5'- aCGAGcGGAUGGCCGacggcUGGGAgcUCGUCu -3' miRNA: 3'- -GCUC-UCUGCUGGUa----GCCCUauAGCGG- -5' |
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| 16332 | 5' | -52.9 | NC_004084.1 | + | 44671 | 0.71 | 0.592784 |
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Target: 5'- ---cGGACGAUacuuguUCGGGAUAUCGUCg -3' miRNA: 3'- gcucUCUGCUGgu----AGCCCUAUAGCGG- -5' |
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| 16332 | 5' | -52.9 | NC_004084.1 | + | 46998 | 0.7 | 0.680273 |
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Target: 5'- --cGAGACGACCGcgaguuccUCGaGGAcGUCGCg -3' miRNA: 3'- gcuCUCUGCUGGU--------AGC-CCUaUAGCGg -5' |
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| 16332 | 5' | -52.9 | NC_004084.1 | + | 47504 | 0.67 | 0.831866 |
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Target: 5'- gCGaAGAcGACGAacuccCCgAUCGGGAguacccgacgAUCGCCa -3' miRNA: 3'- -GC-UCU-CUGCU-----GG-UAGCCCUa---------UAGCGG- -5' |
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| 16332 | 5' | -52.9 | NC_004084.1 | + | 48895 | 0.68 | 0.764791 |
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Target: 5'- -cAGuGAUGACCGUCGGucgGUCgGCCa -3' miRNA: 3'- gcUCuCUGCUGGUAGCCcuaUAG-CGG- -5' |
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| 16332 | 5' | -52.9 | NC_004084.1 | + | 50790 | 0.66 | 0.857689 |
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Target: 5'- cCGAcGAGACGaACC--CGGGA-AUCGUCc -3' miRNA: 3'- -GCU-CUCUGC-UGGuaGCCCUaUAGCGG- -5' |
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| 16332 | 5' | -52.9 | NC_004084.1 | + | 53125 | 0.68 | 0.774866 |
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Target: 5'- cCGAGuggauGGCgGACCAUccgcugCGGGAcGUCGUCg -3' miRNA: 3'- -GCUCu----CUG-CUGGUA------GCCCUaUAGCGG- -5' |
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| 16332 | 5' | -52.9 | NC_004084.1 | + | 55376 | 0.71 | 0.636563 |
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Target: 5'- uCGAGGGugGCGAUCAUCGccGUAUuCGCCa -3' miRNA: 3'- -GCUCUC--UGCUGGUAGCccUAUA-GCGG- -5' |
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| 16332 | 5' | -52.9 | NC_004084.1 | + | 57122 | 0.68 | 0.774866 |
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Target: 5'- uCGAG-GACGuCC-UCGG--UGUCGCCg -3' miRNA: 3'- -GCUCuCUGCuGGuAGCCcuAUAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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