miRNA: 3'- uaCAGCaGC-ACCGCCG-UCaCAGG--CUG- -5'
miRNA: 3'- uaCaGCAGCAC-CGcCGUCACAG--GCUG- -5'
miRNA: 3'- -UACAGCAG---------CACCGCCgu-CACA--GGCUG- -5'
miRNA: 3'- uaCaGCa--GCACCGCCGUCacaGGCUG- -5'
miRNA: 3'- -UACAGCAGCACCGCCGUCACAGGCUG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16336 | 3' | -57.9 | NC_004084.1 | + | 50258 | 0.68 | 0.478242 |
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Target: 5'- -cGUCGcCGuUGGCGGCgAG-GUCCucGACg -3' miRNA: 3'- uaCAGCaGC-ACCGCCG-UCaCAGG--CUG- -5' |
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| 16336 | 3' | -57.9 | NC_004084.1 | + | 56119 | 0.68 | 0.468299 |
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Target: 5'- -cGaCGUCGUGcGCcGCAGUGUCguCGGCg -3' miRNA: 3'- uaCaGCAGCAC-CGcCGUCACAG--GCUG- -5' |
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| 16336 | 3' | -57.9 | NC_004084.1 | + | 8804 | 0.68 | 0.439132 |
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Target: 5'- cAUGUCGUCaucguccagGUGGUGGagcGUGUcgCCGACa -3' miRNA: 3'- -UACAGCAG---------CACCGCCgu-CACA--GGCUG- -5' |
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| 16336 | 3' | -57.9 | NC_004084.1 | + | 11538 | 0.69 | 0.401913 |
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Target: 5'- -cGcCGgggCGUGGCGGCAG---CCGACg -3' miRNA: 3'- uaCaGCa--GCACCGCCGUCacaGGCUG- -5' |
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| 16336 | 3' | -57.9 | NC_004084.1 | + | 14098 | 1.06 | 0.000863 |
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Target: 5'- gAUGUCGUCGUGGCGGCAGUGUCCGACc -3' miRNA: 3'- -UACAGCAGCACCGCCGUCACAGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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