Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16342 | 3' | -54.1 | NC_004084.1 | + | 10548 | 0.66 | 0.763006 |
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Target: 5'- cCAgCGGACGaguagcUCGGGGuGGCUGUc -3' miRNA: 3'- aGUaGCUUGCaa----AGCCCCuCCGGCAa -5' |
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| 16342 | 3' | -54.1 | NC_004084.1 | + | 25182 | 0.67 | 0.752677 |
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Target: 5'- cUCAUCGAACucguaGGGGAcGGCCc-- -3' miRNA: 3'- -AGUAGCUUGcaaagCCCCU-CCGGcaa -5' |
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| 16342 | 3' | -54.1 | NC_004084.1 | + | 9247 | 0.67 | 0.710236 |
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Target: 5'- gCGUCcAGCGUcUCGGGGucGUCGUUc -3' miRNA: 3'- aGUAGcUUGCAaAGCCCCucCGGCAA- -5' |
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| 16342 | 3' | -54.1 | NC_004084.1 | + | 35868 | 0.68 | 0.644471 |
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Target: 5'- -aGUCGAACaucgaaGGGGAGGCCa-- -3' miRNA: 3'- agUAGCUUGcaaag-CCCCUCCGGcaa -5' |
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| 16342 | 3' | -54.1 | NC_004084.1 | + | 18547 | 0.7 | 0.567338 |
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Target: 5'- gCAUCGAAC---UCGGGuGGGCCGa- -3' miRNA: 3'- aGUAGCUUGcaaAGCCCcUCCGGCaa -5' |
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| 16342 | 3' | -54.1 | NC_004084.1 | + | 17994 | 0.7 | 0.545649 |
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Target: 5'- -uGUCGAGCGUUcgugaaggucucUCGGGGGuGCUGUa -3' miRNA: 3'- agUAGCUUGCAA------------AGCCCCUcCGGCAa -5' |
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| 16342 | 3' | -54.1 | NC_004084.1 | + | 11643 | 1.06 | 0.002168 |
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Target: 5'- cUCAUCGAACGUUUCGGGGAGGCCGUUa -3' miRNA: 3'- -AGUAGCUUGCAAAGCCCCUCCGGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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