Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16344 | 3' | -56.8 | NC_004084.1 | + | 18020 | 0.66 | 0.703725 |
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Target: 5'- -gGGGUGCUgUaGAUgGGGGAGaUCa -3' miRNA: 3'- agCCCAUGAgGgCUAgCCCCUCaAGc -5' |
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| 16344 | 3' | -56.8 | NC_004084.1 | + | 56604 | 0.66 | 0.693222 |
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Target: 5'- gCGGcGUcgUCgCCGGUCGGcGAGUUCGc -3' miRNA: 3'- aGCC-CAugAG-GGCUAGCCcCUCAAGC- -5' |
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| 16344 | 3' | -56.8 | NC_004084.1 | + | 27946 | 0.66 | 0.682663 |
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Target: 5'- cUCGGGUAa--CUGGUCGGGG--UUCa -3' miRNA: 3'- -AGCCCAUgagGGCUAGCCCCucAAGc -5' |
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| 16344 | 3' | -56.8 | NC_004084.1 | + | 28290 | 0.66 | 0.682663 |
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Target: 5'- aCGGGaguCUCgCGAUCGGGGcgg-CGa -3' miRNA: 3'- aGCCCau-GAGgGCUAGCCCCucaaGC- -5' |
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| 16344 | 3' | -56.8 | NC_004084.1 | + | 54499 | 0.66 | 0.662484 |
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Target: 5'- gUCGGGUGCg-CCGuaguggggguacagCGGGGAGUg-- -3' miRNA: 3'- -AGCCCAUGagGGCua------------GCCCCUCAagc -5' |
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| 16344 | 3' | -56.8 | NC_004084.1 | + | 6796 | 0.67 | 0.62297 |
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Target: 5'- gUCGGGgaucaGCUCCCGcgccgucgacgucauAUCGuuGAGUUCGu -3' miRNA: 3'- -AGCCCa----UGAGGGC---------------UAGCccCUCAAGC- -5' |
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| 16344 | 3' | -56.8 | NC_004084.1 | + | 33005 | 0.68 | 0.54466 |
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Target: 5'- cUCGGGa--UgCgGAUCGGGGAGaUCGc -3' miRNA: 3'- -AGCCCaugAgGgCUAGCCCCUCaAGC- -5' |
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| 16344 | 3' | -56.8 | NC_004084.1 | + | 18555 | 0.69 | 0.52398 |
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Target: 5'- cUCGGGUGgg-CCGA-CGGGGAGaUCGc -3' miRNA: 3'- -AGCCCAUgagGGCUaGCCCCUCaAGC- -5' |
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| 16344 | 3' | -56.8 | NC_004084.1 | + | 20937 | 0.7 | 0.435315 |
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Target: 5'- uUCGGGUAUcuUCCCGAccgugucacgUCGGGG---UCGg -3' miRNA: 3'- -AGCCCAUG--AGGGCU----------AGCCCCucaAGC- -5' |
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| 16344 | 3' | -56.8 | NC_004084.1 | + | 10902 | 1.09 | 0.001014 |
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Target: 5'- gUCGGGUACUCCCGAUCGGGGAGUUCGu -3' miRNA: 3'- -AGCCCAUGAGGGCUAGCCCCUCAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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